Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewSAG
说明： Potent Smoothened receptor agonist; activates the Hedgehog signaling pathway
化学名： 3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide
纯度： ≥98% (HPLC)
生物活性 for SAG
SAG is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM) and induces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons.
SAG dihydrochloride (Cat. No. 6390) also available.
化合物库 for SAG
SAG is also offered as part of the Tocriscreen Stem Cell Library. 了解 Tocris 化合物库的更多信息。
技术数据 for SAG
| 分子量 | 490.06 |
| 公式 | C28H28ClN3OS |
| 储存 | Store at -20°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 912545-86-9 |
| PubChem ID | 5284330 |
| InChI Key | VFSUUTYAEQOIMW-UHFFFAOYSA-N |
| Smiles | ClC2=C(C(N([C@H]4CC[C@H](NC)CC4)CC3=CC=CC(C5=CC=NC=C5)=C3)=O)SC1=CC=CC=C12 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for SAG
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 49.01 | 100 |
制备储备液 for SAG
以下数据基于产品分子量 490.06。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.04 mL | 10.2 mL | 20.41 mL |
| 5 mM | 0.41 mL | 2.04 mL | 4.08 mL |
| 10 mM | 0.2 mL | 1.02 mL | 2.04 mL |
| 50 mM | 0.04 mL | 0.2 mL | 0.41 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
产品说明书 for SAG
参考文献 for SAG
参考文献是支持产品生物活性的出版物。
Stanton et al (2009) A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat.Chem.Biol. 5 154 PMID: 19151731
Chen et al (2002) Small molecule modulation of Smoothened activity. Proc.Natl.Acad.Sci.USA. 99 14071 PMID: 12391318
Mak et al (2012) Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage. Stem Cells Int. 2012 140427 PMID: 22567022
If you know of a relevant reference for SAG, please let us know.
按标靶查看相关产品
按产品操作查看相关产品
关键词: SAG, SAG supplier, Potent, Smoothened, Smo, receptors, agonists, activates, Hedgehog, signaling, pathway, activator, enhancers, neuronal, differentiation, iPCs, inducible, pluripotent, stem, cells, dopaminergic, neurons, neurones, Receptors, Signaling, Neural, Stem, Cells, ESCs, and, iPSC, 4366, Tocris Bioscience
21 篇 SAG 的引用文献
引用文献是使用了 Tocris 产品的出版物。 SAG 的部分引用包括：
Murase et al (2024) In vitro reconstitution of epigenetic reprogramming in the human germ line. Nature PMID: 38768632
Xin et al (2023) Characterizing the Neuron-Glial Interactions by the Co-cultures of Human iPSC-Derived Oligodendroglia and Neurons. Methods Mol Biol 2683 103-111 PMID: 37300770
Sundari et al (2020) Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions. J Vis Exp PMID: 33226027
Amos et al (2020) SEM/FIB Imaging for Studying Neural Interfaces. Dev Neurobiol 80 305-315 PMID: 31228876
Frédéric H-T et al (2020) Aberrant interaction of FUS with the U1 snRNA provides a molecular mechanism of FUS induced amyotrophic lateral sclerosis. Nat Commun 11 6341 PMID: 33311468
Alon et al (2020) Electrophysiologic Characterization of Developing Human Embryonic Stem Cell-Derived Photoreceptor Precursors. Invest Ophthalmol Vis Sci 61 44 PMID: 32991686
Shoji et al (2020) Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis. J Cell Biol 219 PMID: 32211891
Carel B et al (2020) Detailed Phenotyping and Therapeutic Strategies for Intronic ABCA4 Variants in Stargardt Disease. Mol Ther Nucleic Acids 21 412-427 PMID: 32653833
Xia et al (2016) Transcriptional comparison of human induced and primary midbrain DArgic neurons. Sci.Rep. 6 20270 PMID: 26842779
Atzmon et al (2018) Drug Screening Identifies Sigma-1-Receptor as a Target for the Therapy of VWM Leukodystrophy. Front Mol Neurosci 11 336 PMID: 30279648
Cortes et al (2015) Accumulation of the Vitamin D Precursor Cholecalciferol Antagonizes Hedgehog Signaling to Impair Hemogenic Endothelium Formation. Nat Chem Biol 5 471 PMID: 26365513
Ruili et al (2021) Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules. Methods Mol Biol 2454 811-827 PMID: 34128205
Beth A et al (2021) Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging. Commun Biol 4 786 PMID: 34168275
Ludo et al (2021) Induced pluripotent stem cell-derived motor neurons of CMT type 2 patients reveal progressive mitochondrial dysfunction. Brain 144 2471-2485 PMID: 34128983
John M et al (2021) A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins. EMBO J 40 e103811 PMID: 33644875
Anestis et al (2021) In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells. Curr Protoc 1 e244 PMID: 34547185
Haidar et al (2019) Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies. Autophagy 15 1051 PMID: 30669930
Aaron C et al (2022) Primary cilia on muscle stem cells are critical to maintain regenerative capacity and are lost during aging. Nat Commun 13 1439 PMID: 35301320
Eva et al (2019) Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes. Bio Protoc 9 e3312 PMID: 33654821
Massimiliano et al (2019) Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration. Autophagy 15 631-651 PMID: 30335591
Rajamani et al (2018) Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses. Cell Stem Cell 22 698 PMID: 29681516
您是否知道使用了 Tocris SAG 的优秀论文？ 请告知我们.
SAG 的评论
平均评分: 5 (基于 2 条评论。)
Have you used SAG?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Promoted the formation of cilia.
By
Kishor Pant on 02/10/2023
分析类型： In Vitro
种属： Human
细胞系/组织： HepG2, and Huh,
SAG consistently produced positive results in our experiments; it promoted the formation of cilia and reduced the proliferation of cancer cells.
We use 5-20 nm concentration for 24-48 hours.
Usage Information.
By
Anonymous on 07/04/2020
分析类型： In Vitro
种属： Mouse
细胞系/组织： iPSC
iPSC-derived hypothalamic neuron differentiation activation by Smoothened agonist SAG 1 μM and purmorphamine 1 μM.
From Day 2 to day 9 to direct the cells toward ventral diencephalon with regular media change every 2 days.
该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
- Self-renewal and Maintenance
- Differentiation
- Reprogramming
- Organoid Generation
- GMP and Ancillary Material Grade Products