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sCy5DL-amide

Cat. No. 7835 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： FDAA for super-resolution microscopy of bacteria
化学名： 2-((1E,3E)-5-((E)-1-(6-(((R)-6-Amino-5-ammonio-6-oxohexyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)penta-1,3-dien-1-yl)-1,3,3-trimethyl-3H-indol-1-ium-5-sulfonate
纯度： ≥95% (HPLC)
说明书
引用文献
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文献 (1)
生物活性 Scientific Data 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for sCy5DL-amide

sCy5DL-amide is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into Gram-positive B.subtilis. sCy5DL-amide labeled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/666 nm.

Scientific Data

Fluorescence Imaging Bacillus subtilis cells stained with sCy5DA-amide View Larger

Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA-amide

Optical Data for sCy5DL-amide

sCy5DL-amide Dye Spectra
Emission Color Red
λabs 646 nm
λem 666 nm
Extinction Coefficient (ε) 209,200 M-1cm-1
Cell Permeable Yes
Application STORM

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for sCy5DL-amide

分子量 769.97
公式 C38H51N5O8S2
储存 Store at -20°C
纯度 ≥95% (HPLC)
InChI Key KASKHZOOXRBMET-SSEXGKCCSA-N
Smiles CC1(C2=C(N(/C1=C/C=C/C=C/C3=[N+](C)C4=C(C=C(C=C4)S(=O)([O-])=O)C3(C)C)CCCCCC(NCCCC[C@@H]([NH3+])C(N)=O)=O)C=CC(S(=O)([O-])=O)=C2)C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for sCy5DL-amide

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 7.7 10

制备储备液 for sCy5DL-amide

以下数据基于产品分子量 769.97。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 12.99 mL 64.94 mL 129.88 mL
0.5 mM 2.6 mL 12.99 mL 25.98 mL
1 mM 1.3 mL 6.49 mL 12.99 mL
5 mM 0.26 mL 1.3 mL 2.6 mL

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参考文献 for sCy5DL-amide

参考文献是支持产品生物活性的出版物。

Zhang et al (2022) Fluorescent D-amino acids for super-resolution microscopy of the bacterial cell wall. ACS Chem.Biol. 117 2418 PMID: 35994360

If you know of a relevant reference for sCy5DL-amide, please let us know.

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关键词: sCy5DL-amide, sCy5DL-amide supplier, fluorescent, D-amino, acid, FDAA, peptidoglycans, labeling, labels, labelling, amino, acids, probes, for, imaging, bacteria, SMLM, PALM, STORM, Fluorescent, Probes, Imaging, Bacteria, 7835, Tocris Bioscience

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