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TSA Vivid™ Fluorophore Kit 650

Cat. No. 7527 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Signal amplification kit for use in ISH, ICC and IHC
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生物活性 for TSA Vivid™ Fluorophore Kit 650

TSA Vivid™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. TSA Vivid reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.

The basis for signal amplification comes from the specific and high-density deposition of TSA Vivid dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).

Excitation maximum = 654 nm; emission maximum = 668 nm

Please see the product datasheet or protocol for information on product use.

TSA Vivid is a trademark of Bio-Techne Corp.

Scientific Data

Application of TSA Vivid dye 650 in RNAscope Multiplex Fluorescent v2 Assay View Larger

Application of TSA Vivid dye 650 in RNAscope™ Multiplex Fluorescent v2 Assay. RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and TSA Vivid dye 650 (right). Dye used at 1:1500 dilution. Marker shown is UBC in white. Nuclei are counterstained with DAPI (blue, Catalog # 5748, Tocris).

Optical Data for TSA Vivid™ Fluorophore Kit 650

Spectral data for TSA Vivid Fluorophore kit 650 Emission Color Red
λabs 654 nm
λem 668 nm

技术数据 for TSA Vivid™ Fluorophore Kit 650

储存 Store at -20°C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

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TSA Vivid™ Fluorophore Kit 650 实验方案

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