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Submit ReviewSCR7 pyrazine
Description: Enhances HDR efficiency
Chemical Name: 2,3-Dihydro-6,7-diphenyl-2-thioxo-4(1H)-pteridinone
Purity: ≥98% (HPLC)
Biological Activity for SCR7 pyrazine
SCR7 pyrazine enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).
We have discovered that the commercially available SCR7 material supplied by other vendors does not match the chemical structure that it is being sold under. Through detailed chemical analysis carried out by our Analytical Quality Control experts, we believe that the commercially available SCR7 material, including that used in recent publications to enhance CRISPR efficiency, is in fact an analog of SCR7 that we have named 'SCR7 pyrazine'.
Technical Data for SCR7 pyrazine
| M. Wt | 332.38 |
| Formula | C18H12N4OS |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 14892-97-8 |
| PubChem ID | 10688007 |
| InChI Key | GSRTWXVBHGOUBU-UHFFFAOYSA-N |
| Smiles | O=C(C(N=C(C3=CC=CC=C3)C(C4=CC=CC=C4)=N2)=C2N1)NC1=S |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for SCR7 pyrazine
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 33.44 | 100 | |
| ethanol | 6.69 | 20 |
Preparing Stock Solutions for SCR7 pyrazine
The following data is based on the product molecular weight 332.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.01 mL | 15.04 mL | 30.09 mL |
| 5 mM | 0.6 mL | 3.01 mL | 6.02 mL |
| 10 mM | 0.3 mL | 1.5 mL | 3.01 mL |
| 50 mM | 0.06 mL | 0.3 mL | 0.6 mL |
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Product Datasheets for SCR7 pyrazine
References for SCR7 pyrazine
References are publications that support the biological activity of the product.
Chu et al (2015) Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat.Biotechnol. 33 543 PMID: 25803306
Maruyama et al (2015) Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Nat.Biotechnol. 33 538 PMID: 25798939
Greco et al (2016) Synthesis and structure determination of SCR7, a DNA ligase inhibitor Teterahedron Lett. 57 3204
Greco et al (2016) SCR7 is neither a selective nor a potent inhibitor of human DNA ligase IV. DNA Repair (Amst) 43 18 PMID: 27235626
If you know of a relevant reference for SCR7 pyrazine, please let us know.
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Keywords: SCR7 pyrazine, SCR7 pyrazine supplier, CRISPR, Cas9, HDR, NHEJ, homology, directed, repair, nonhomologous, end, joining, hematopoietic, haematopoietic, stem, cells, HSC, Reagents, Hematopoietic, Stem, Cells, 5342, Tocris Bioscience
2 Citations for SCR7 pyrazine
Citations are publications that use Tocris products. Selected citations for SCR7 pyrazine include:
Lei et al (2021) Dynamics and competition of CRISPR-Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing. Nucleic Acids Res 49 969-985 PMID: 33398341
John W et al (2020) RTP4 Is a Potent IFN-Inducible Anti-flavivirus Effector Engaged in a Host-Virus Arms Race in Bats and Other Mammals. Cell Host Microbe 28 712-723.e9 PMID: 33113352
Do you know of a great paper that uses SCR7 pyrazine from Tocris? Please let us know.
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