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Biotinyl Tyramide

Cat. No. 6241 2 Citations 2 Reviews Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Reagent widely used for signal amplification in IHC and FISH
Chemical Name: (3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
Purity: ≥98% (HPLC)
Datasheet
Citations (2)
Reviews (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Biotinyl Tyramide

Biotinyl Tyramide is a reagent widely used for signal amplification in IHC and fluorescent in situ hybridization (ISH) and APEX proximity labeling. HRP catalyzes localized deposition of multiple biotinyl tyramide molecules (catalyzed reporter deposition, CARD), which can be detected using a labeled streptavidin conjugate. The localization of deposition around HRP leads to good resolution. Suitable for either fluorescence or chromogenic detection. Biotinyl Tyramide is used as a substrate for APEX proximity labeling.

Technical Data for Biotinyl Tyramide

M. Wt 363.47
Formula C18H25N3O3S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 41994-02-9
PubChem ID 21826423
InChI Key VZWXNOBHWODXCW-ZOBUZTSGSA-N
Smiles [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCC3=CC=C(C=C3)O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Biotinyl Tyramide

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 36.35 100

Preparing Stock Solutions for Biotinyl Tyramide

The following data is based on the product molecular weight 363.47. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.75 mL 13.76 mL 27.51 mL
5 mM 0.55 mL 2.75 mL 5.5 mL
10 mM 0.28 mL 1.38 mL 2.75 mL
50 mM 0.06 mL 0.28 mL 0.55 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

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Product Datasheets for Biotinyl Tyramide

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for Biotinyl Tyramide

References are publications that support the biological activity of the product.

Bobrow et al (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 125 279 PMID: 2558138

McKay et al (1997) Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide. Mol.Pathol. 50 322 PMID: 9536283

If you know of a relevant reference for Biotinyl Tyramide, please let us know.

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Keywords: Biotinyl Tyramide, Biotinyl Tyramide supplier, in, situ, hybridization, ISH, immunohistochemistry, IHC, fluorescence, fluorescent, tyramide, signal, amplification, APEX, proximity, labeling, Tyramide, Signaling, Amplification, (TSA), 6241, Tocris Bioscience

2 Citations for Biotinyl Tyramide

Citations are publications that use Tocris products. Selected citations for Biotinyl Tyramide include:

Jonas et al (2022) The Development of Hindlimb Postural Asymmetry Induced by Focal Traumatic Brain Injury Is Not Related to Serotonin 2A/C Receptor Expression in the Spinal Cord. Int J Mol Sci 23 PMID: 35628167

Nicholas T et al (2022) Proximity-based labeling reveals DNA damage-induced phosphorylation of fused in sarcoma (FUS) causes distinct changes in the FUS protein interactome. J Biol Chem 298 102135 PMID: 35709984

Do you know of a great paper that uses Biotinyl Tyramide from Tocris? Please let us know.

Reviews for Biotinyl Tyramide

Average Rating: 5 (Based on 2 Reviews.)

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Biotinyl tyramide used successfully for BP-labelling of mitochondrial proteins.
By Anonymous on 02/12/2020
Assay Type: In Vitro
Species: Human

BP-labelling experiment in SH-SY5Y cells stably expressing MTS-APEX2. Tested range 500-1000µM biotinyl tyramide.

review image
Great product for Biotinylation.
By Anonymous on 02/07/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: SH-SY5Y

We use biotinyl tyramide to biotinylate cells - we have done this successfully without any issues to date. Biotinylated proteins can be detected by western blot using a streptavidin conjugated antibody (see image)

review image