Home / Product Type / Fluorescence Imaging / Fluorescent Probes and Stains / Fluorescent Receptor Probes / CELT-327

CELT-327

Cat. No. 7954 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Potent and selective hA2B/A3 adenosine receptor fluorescent antagonist
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (2)
Biological Activity Scientific Data Technical Data Datasheets References

Biological Activity for CELT-327

CELT-327 is a potent and selective fluorescent hA2B/A3 adenosine receptor antagonist (Ki values are 35.6 nM and 45.7 nM at A2B and A3 receptors respectively). It shows dose-dependent membrane fluorescent labeling in HCT116 cells expressing A2B adenosine receptors, with rapid diffusion and homogeneous distribution of the fluorescence signal within the cell monolayer. In live HCT116 compact spheroids, CELT-327 displays homogeneous staining across the entire spheroid diameter, reaching deep spheroid regions. CELT-327 can be used as a fluorescent adenosine receptor probe for fluorescence binding assays, live cell imaging, flow cytometry, and fluorescence polarization assays. It is a suitable replacement for GPCR radiolabeling ligands. Excitation and emission maxima (λ) are 589 nm and 616 nm, respectively. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.

Add 75 μL of assay buffer containing 1% of DMSO to obtain a 100 μM stock solution, and use 250 nM as a working concentration.

Scientific Data

Live-cell Imaging Application of CELT-327 to live colorectal cancer cells View Larger

Application of CELT-327 to live colorectal cancer cells. HeLa cells were co-cultured with fibroblasts from the stroma around tumors (CAFs) previously labeled in green with a cell-tracker. Both cell types were incubated with CELT-327 (250 nM). The figure shows in red the plasma membrane staining revealed by CELT-327 specifically in HeLa cells.

Fixed-cell Imaging Application of CELT-327 to fixed colorectal cancer cells. View Larger

Application of CELT-327 to fixed colorectal cancer cells. HCT116 cells were fixed with PFA 4% (20 mins at room temperature) and then incubated with CELT-327 (250 nM) for 30 mins at room temperature, washed and visualized immediately (A), or 96h post-staining (B).

Licensing Information

Sold under license from Celtarys Research

Optical Data for CELT-327

Emission Color Orange
λabs 589 nm
λem 616 nm
Closest Laser line 561 nm
Application Confocal microscopy, High-content screening

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for CELT-327

M. Wt 1334.19
Storage Store at -20°C
Purity ≥95% (HPLC)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

References for CELT-327

References are publications that support the biological activity of the product.

Barbazán et al (2022) Identification of A2BAR as a potential target in colorectal cancer using novel fluorescent GPCR ligands. Biomed.Pharmacother. 153 113408 PMID: 36076535

If you know of a relevant reference for CELT-327, please let us know.

View Related Products by Target

Keywords: CELT-327, CELT-327 supplier, CELT327, potent, selective, A2B, hA2B, A3, Adenosine, receptor, antagonist, fluorescent, probe, fluorescence, live, cell, imaging, spheroids, Fluorescent, Receptor, Probes, TR-FRET, and, FP, Assay, Reagents, Receptors, 7954, Tocris Bioscience

Citations for CELT-327

Citations are publications that use Tocris products.

Currently there are no citations for CELT-327. Do you know of a great paper that uses CELT-327 from Tocris? Please let us know.

Reviews for CELT-327

There are currently no reviews for this product. Be the first to review CELT-327 and earn rewards!

Have you used CELT-327?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals
Enabling Research By Provision of Chemical Tools Poster

Enabling Research By Provision of Chemical Tools Poster

The Tocris chemistry team has considerable chemistry knowledge and skill which it has recently been applying to the generation of new chemical probes from known tools and compounds to help answer biological questions. This poster presents some of the work carried out by Tocris scientists and focuses on two areas: the generation of libraries of chemical building blocks to support Degrader (PROTAC) research and the development of a fluorescent probe to study integrin biology. Presented at Chemical Tools for Complex Biological Systems II, 2019, Janelia Research Campus, USA.