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CELT-426

Cat. No. 7955 Submit a Review Datasheet / COA / SDS
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Description: Potent and partially selective hD2 dopamine receptor fluorescent antagonist
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (2)
Biological Activity Scientific Data Technical Data Datasheets References

Biological Activity for CELT-426

CELT-426 is a potent and partially selective fluorescent hD2 dopamine receptor antagonist (Ki values are 89.3 nM, 194.8 nM, and 263.6 nM for D2, D3, and D4 dopamine receptors respectively in radioligand binding assay). CELT-426 can be used as a fluorescent dopamine receptor probe for fluorescence binding assays, live cell imaging, flow cytometry, and fluorescence polarization assays. It is a suitable replacement for GPCR radiolabeling ligands. Excitation and emission maxima (λ) are 560 and 571 nm, respectively. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.

Add 62 μL of assay buffer containing 1% of DMSO to obtain a 100 μM stock solution, and use 250 nM as working concentration.

Scientific Data

Competition Assay Application of CELT-426 in high content screening assay. View Larger

Application of CELT-426 in high content screening assay. Competition binding assay between CELT-426 (100 nM) and increasing concentrations of haloperidol (Catalog # 0931) in live adherent CHO cells expressing hD2.

Application of CELT-426 in fluorescence polarization assay. View Larger

Application of CELT-426 in fluorescence polarization assay. Human dopamine D2 receptor was isolated from CHO cells by membrane preparation and incubated with CELT-426 (100 nM). Haloperidol (Catalog # 0931) was added across a range of concentrations, and the binding affinity (KD = 3 nM) determined by fluorescence polarization.

Licensing Information

Sold under license from Celtarys Research

Optical Data for CELT-426

Emission Color Yellow
λabs 560 nm
λem 571 nm
Closest Laser line 561 nm
Application Fluorescence polarization, Confocal microscopy, Flow Cytometry, High-content screening,

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for CELT-426

M. Wt 1615.85
Storage Store at -20°C
Purity ≥95% (HPLC)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

References for CELT-426

References are publications that support the biological activity of the product.

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Citations for CELT-426

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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Enabling Research By Provision of Chemical Tools Poster

Enabling Research By Provision of Chemical Tools Poster

The Tocris chemistry team has considerable chemistry knowledge and skill which it has recently been applying to the generation of new chemical probes from known tools and compounds to help answer biological questions. This poster presents some of the work carried out by Tocris scientists and focuses on two areas: the generation of libraries of chemical building blocks to support Degrader (PROTAC) research and the development of a fluorescent probe to study integrin biology. Presented at Chemical Tools for Complex Biological Systems II, 2019, Janelia Research Campus, USA.