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Description: Orange fluorescent reagent widely used for signal amplification in IHC and FISH
Chemical Name: 2-[3-[(1-Ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt
Purity: ≥95% (HPLC)
Biological Activity for Cyanine 3 Tyramide
Cyanine 3 Tyramide is an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.
Optical Data for Cyanine 3 Tyramide
| Emission Color | Orange |
|---|---|
| λabs | 550 nm |
| λem | 563 nm |
| Extinction Coefficient (ε) | 150000 M-1cm-1 |
| Quantum Yield (φ) | 0.31 |
| Closest Laser line | 532 nm |
| Application | Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Cyanine 3 Tyramide
| M. Wt | 749.94 |
| Formula | C39H47N3O8S2 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 174961-75-2 |
| InChI Key | JPXIOSIRIWBDIC-UHFFFAOYSA-N |
| Smiles | CCN1C2=C(C=C(C=C2)S(O)(=O)=O)C(/C1=C\C=C\C3=[N+](CCCCCC(NCCC4=CC=C(O)C=C4)=O)C5=C(C=C(C=C5)S([O-])(=O)=O)C3(C)C)(C)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Cyanine 3 Tyramide
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 12 | 16 |
Preparing Stock Solutions for Cyanine 3 Tyramide
The following data is based on the product molecular weight 749.94. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.16 mM | 8.33 mL | 41.67 mL | 83.34 mL |
| 0.8 mM | 1.67 mL | 8.33 mL | 16.67 mL |
| 1.6 mM | 0.83 mL | 4.17 mL | 8.33 mL |
| 8 mM | 0.17 mL | 0.83 mL | 1.67 mL |
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Product Datasheets for Cyanine 3 Tyramide
References for Cyanine 3 Tyramide
References are publications that support the biological activity of the product.
Schmidt et al (1997) Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J.Histochem.Cytochem. 45 365 PMID: 9071318
van Gijlswijk et al (1997) Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 45 375 PMID: 9071319
Hopman et al (1998) Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 46 771 PMID: 9603790
Schriml et al (1999) Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 27 608 PMID: 10489619
Zhao et al (2013) Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization and immunohistochemical study using tyramide signal amplification. PLoS One 8 e73750 PMID: 23967349
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Keywords: Cyanine 3 Tyramide, Cyanine 3 Tyramide supplier, C3, tyramide, signal, amplification, TSA, horseradish, peroxidase, HRP, orange, Tyramide, Signaling, Amplification, (TSA), 6457, Tocris Bioscience
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