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Description: Red fluorescent reagent widely used for signal amplification in IHC and FISH
Alternative Names: Cy5 Tyramide
Chemical Name: 2-[5-[1,3-Dihydro-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino]-6-oxohexyl]3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium inner salt
Purity: ≥95% (HPLC)
Biological Activity for Cyanine 5 Tyramide
Cyanine 5 Tyramide is a red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.
Optical Data for Cyanine 5 Tyramide
| Emission Color | Red |
|---|---|
| λabs | 651 nm |
| λem | 665 nm |
| Extinction Coefficient (ε) | 250000 M-1cm-1 |
| Closest Laser line | 647 nm |
| Application | Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH |
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Technical Data for Cyanine 5 Tyramide
| M. Wt | 775.97 |
| Formula | C41H49N3O8S2 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 1431148-26-3 |
| PubChem ID | 135290897 |
| InChI Key | NHSJCIBSNKLAMA-UHFFFAOYSA-N |
| Smiles | CC[N+]1=C(C(C)(C2=CC(S([O-])(=O)=O)=CC=C12)C)/C=C/C=C/C=C3N(C4=CC=C(S(O)(=O)=O)C=C4C/3(C)C)CCCCCC(NCCC5=CC=C(C=C5)O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Cyanine 5 Tyramide
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.64 | 15 |
Preparing Stock Solutions for Cyanine 5 Tyramide
The following data is based on the product molecular weight 775.97. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.15 mM | 8.59 mL | 42.96 mL | 85.91 mL |
| 0.75 mM | 1.72 mL | 8.59 mL | 17.18 mL |
| 1.5 mM | 0.86 mL | 4.3 mL | 8.59 mL |
| 7.5 mM | 0.17 mL | 0.86 mL | 1.72 mL |
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Product Datasheets for Cyanine 5 Tyramide
References for Cyanine 5 Tyramide
References are publications that support the biological activity of the product.
Chanda et al (2017) Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer. PLoS One 12 e0177639 PMID: 28493978
Matsumoto et al (2017) Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer. Br.J.Cancer 117 1537 PMID: 28881356
Wong et al (2001) Identification of differentially expressed genes from ovarian cancer cells by MICROMAX cDNA microarray system. Biotechniques 30 670 PMID: 11252802
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Keywords: Cyanine 5 Tyramide, Cyanine 5 Tyramide supplier, Cy5, tyramide, signal, amplification, TSA, horseradish, peroxidase, HRP, red, Tyramide, Signaling, Amplification, (TSA), 6458, Tocris Bioscience
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