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Submit ReviewDihydroethidium
Discontinued Product
7642 has been discontinued.
View all Fluorescent Cell Indicators and Sensors products.
Description: Fluorescent ROS indicator
Chemical Name: 5-Ethyl-5,6-dihydro-6-phenyl-3,8-phenanthridinediamine
Purity: ≥95% (HPLC)
Biological Activity for Dihydroethidium
Dihydroethidium is a fluorescent reactive oxygen species indicator. Cell permeable and suitable for use in live cells. Can be used with Mito-HE (Cat. No. 7641) to measure superoxide in living cells. Excitation/emission maxima λ 350/595 nm
Optical Data for Dihydroethidium
| Emission Color | Yellow |
|---|---|
| λabs | 500 nm |
| λem | 582 nm |
| Extinction Coefficient (ε) | 14000 M-1cm-1 |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Confocal Imaging |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Dihydroethidium
| M. Wt | 315.41 |
| Formula | C21H21N3 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 104821-25-2 |
| PubChem ID | 128682 |
| InChI Key | XYJODUBPWNZLML-UHFFFAOYSA-N |
| Smiles | NC=1C=CC=2C3=CC=C(N)C=C3C(C=4C=CC=CC4)N(C2C1)CC |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Dihydroethidium
References for Dihydroethidium
References are publications that support the biological activity of the product.
Peterson et al (2002) Hydroethidine detection of superoxide production during the lithium-pilocarpine model of status epilepticus. Epilepsy Res. 49 226 PMID: 12076844
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Keywords: Dihydroethidium, Dihydroethidium supplier, hydroethidium, ethidium, ROS, reactive, oxygen, species, superoxide, indicator, Fluorescent, Cell, Indicators, and, Sensors, 7642, Tocris Bioscience
Citations for Dihydroethidium
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals