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Description: Fluorescent probe for labeling mutant FKBP12 (F36V) proteins
Chemical Name: (R)-1-(2-(2-((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)amino)-2-oxoethoxy)phenyl)-3-(3,4-dimethoxyphenyl)propyl (S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate
Purity: ≥95% (HPLC)
Biological Activity for dTAG-Fluorescein
dTAG-Fluorescein is a fluorescent probe for labeling FKBP12F36V fusion proteins. Comprises a high-affinity ligand for FKBP12F36V mutant protein (Kd in the subnanomolar range and 1,000-fold selectivity for the F36V mutant of FKBP12 over wild-type) coupled to 5-amino fluorescein. dTAG-Fluorescein can fluorescently label FKBP12F36V fusion proteins expressed at different levels in a variety of cell lines.
Optical Data for dTAG-Fluorescein
| Emission Color | Green |
|---|---|
| λabs | 493 nm |
| λem | 514 nm |
| Extinction Coefficient (ε) | 68800 M-1cm-1 |
| Closest Laser line | 488 nm |
| Application | Confocal microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for dTAG-Fluorescein
| M. Wt | 1023.1 |
| Formula | C58H58N2O15 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | ZSRZNOZTAUPBBF-ZSDGZGBPSA-N |
| Smiles | CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NC4=CC(C(O5)=O)=C(C=C4)C65C7=C(C=C(C=C7)O)OC8=C6C=CC(O)=C8)=O)CCC9=CC(OC)=C(C=C9)OC)=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for dTAG-Fluorescein
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 10.23 | 10 |
Preparing Stock Solutions for dTAG-Fluorescein
The following data is based on the product molecular weight 1023.1. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 9.77 mL | 48.87 mL | 97.74 mL |
| 0.5 mM | 1.95 mL | 9.77 mL | 19.55 mL |
| 1 mM | 0.98 mL | 4.89 mL | 9.77 mL |
| 5 mM | 0.2 mL | 0.98 mL | 1.95 mL |
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Product Datasheets for dTAG-Fluorescein
References for dTAG-Fluorescein
References are publications that support the biological activity of the product.
Nabet et al (2018) The dTAG system for immediate and target-specific protein degradation. Nat.Chem.Biol. 14 431 PMID: 29581585
Marks et al (2004) A general approach for chemical labeling and rapid, spatially controlled protein inactivation. Proc.Natl.Acad.Sci.U.S.A. 101 9982 PMID: 15218100
If you know of a relevant reference for dTAG-Fluorescein, please let us know.
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Keywords: dTAG-Fluorescein, dTAG-Fluorescein supplier, chemical, labeling, probe, FKBP12(F36V), dTAG, fluorescein, fluorescence, microscopy, TAG, Degradation, Platform, Other, Fluorescent, Probes, 7892, Tocris Bioscience
Citations for dTAG-Fluorescein
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Literature in this Area
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TPD and Induced Proximity Research Product GuideUpdated
This brochure highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation and Induced Proximity research, including:
- Active Degraders
- TAG Degradation Platform
- Degrader Building Blocks
- Assays for Protein Degradation
- Induced Proximity Tools
Targeted Protein Degradation Poster
Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia
