dTAG^V-1 hydrochloride

Name: dTAG V -1 hydrochloride
Brand: Tocris Bioscience
SKU: 7374
Rating: 5 (1 reviews)

Cat. No. 7374 1 Review Submit a Review Datasheet / COA / SDS

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Description: Hydrochloride salt of dTAG^V-1 (Cat. No. 6914); suitable for in vivo use

Chemical Name: (R)-3-(3,4-Dimethoxyphenyl)-1-(2-(2-((7-(((S)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-7-oxoheptyl)amino)-2-oxoethoxy)phenyl)propyl (S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate hydrochloride

Purity: ≥98% (HPLC)

Datasheet

Citations

Reviews (1)

Literature (3)

Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for dTAG^V-1 hydrochloride

dTAG^V-1 hydrochloride is a hydrochloride salt of dTAG^V-1 (Cat. No. 6914). Suitable for use in vivo. Following ip administration of 10 mg/kg in mice: T_½ = 4.43 h; C_max = 2123 ng mL^-1; AUC_inf = 18517 hr*ng mL^-1 and CL = 9.05 mL min^-1 kg^-1

Negative control dTAG^V-1-NEG (Cat. No. 6915) also available.

Important: It is recommended that DMSO stock solutions of this compound are made and used on the same day and are not subjected to freeze/thaw.

Licensing Information

Sold under license from Dana-Farber Cancer Institute

Technical Data for dTAG^V-1 hydrochloride

M. Wt	1284.01
Formula	C₆₈H₉₀N₆O₁₄S.HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2624313-16-0
InChI Key	WZEDGWVEEAWMSD-LNVAYBNASA-N
Smiles	C[C@H](NC([C@@H]1C[C@@H](O)CN1C([C@@H](NC(CCCCCCNC(COC2=CC=CC=C2[C@H](OC([C@@H]3CCCCN3C([C@@H](CC)C4=CC(OC)=C(OC)C(OC)=C4)=O)=O)CCC5=CC(OC)=C(OC)C=C5)=O)=O)C(C)(C)C)=O)=O)C6=CC=C(C=C6)C7=C(N=CS7)C.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for dTAG^V-1 hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	128.4	100

Preparing Stock Solutions for dTAG^V-1 hydrochloride

The following data is based on the product molecular weight 1284.01. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	0.78 mL	3.89 mL	7.79 mL
5 mM	0.16 mL	0.78 mL	1.56 mL
10 mM	0.08 mL	0.39 mL	0.78 mL
50 mM	0.02 mL	0.08 mL	0.16 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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Product Datasheets for dTAG^V-1 hydrochloride

Certificate of Analysis / Product Datasheet

Safety Datasheet

References for dTAG^V-1 hydrochloride

References are publications that support the biological activity of the product.

Nabet et al (2020) Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules. Nat.Commun. 11 4687 PMID: 32948771

Abuhashem et al (2022) Generation of knock-in degron tags for endogenous proteins in mice using the dTAG system. STAR Protoc. 3 101660 PMID: 36097386

If you know of a relevant reference for dTAG^V-1 hydrochloride, please let us know.

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Citations for dTAG^V-1 hydrochloride

Citations are publications that use Tocris products.

Currently there are no citations for dTAG^V-1 hydrochloride. Do you know of a great paper that uses dTAG^V-1 hydrochloride from Tocris? Please let us know.

Reviews for dTAG^V-1 hydrochloride

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Degradation of Myc fusion protein.

By Bikesh Nirala on 06/21/2022

Assay Type: In Vitro

Species: Mouse

Cell Line/Tissue: Osteosarcoma cell lines

I used dTAG-v1 for in-vivo and in-vitro protein degradation and it worked perfectly.

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

TPD and Induced Proximity Research Product GuideUpdated

This brochure highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation and Induced Proximity research, including:

Active Degraders
TAG Degradation Platform
Degrader Building Blocks
Assays for Protein Degradation
Induced Proximity Tools

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia

Validating Targets for TPD Using dTAG Poster

The dTAG platform offers a generalizable strategy to degrade, in principle, any intracellular protein of interest (POI) and is a useful strategy for exploration and validation of targets. This poster presents a workflow solution for target validation, from custom TAG knock-in cell-lines for your POI, different dTAG Degraders to knockdown you POI, to automated assays for protein degradation using Simple Western^TM instruments. Data are also presented on the use of an antibody that recognizes the TAG domain (FKBP12^F36V) for detection of degradation. Presented at TPD Europe, March 2022, London, UK.