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Submit ReviewFCCP
Description: Oxidative phosphorylation uncoupler
Chemical Name: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone
Purity: ≥99% (HPLC)
Biological Activity for FCCP
FCCP is a very potent uncoupler of oxidative phosphorylation in mitochondria.
Technical Data for FCCP
| M. Wt | 254.17 |
| Formula | C10H5F3N4O |
| Storage | Store at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 370-86-5 |
| PubChem ID | 3330 |
| InChI Key | BMZRVOVNUMQTIN-UHFFFAOYSA-N |
| Smiles | N#CC(C#N)=NNC1=CC=C(OC(F)(F)F)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for FCCP
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 25.42 | 100 |
Preparing Stock Solutions for FCCP
The following data is based on the product molecular weight 254.17. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.93 mL | 19.67 mL | 39.34 mL |
| 5 mM | 0.79 mL | 3.93 mL | 7.87 mL |
| 10 mM | 0.39 mL | 1.97 mL | 3.93 mL |
| 50 mM | 0.08 mL | 0.39 mL | 0.79 mL |
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Product Datasheets for FCCP
References for FCCP
References are publications that support the biological activity of the product.
Connop et al (1999) Novel effects of FCCP [carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone] on amyloid precursor protein processing. J.Neurochem. 72 1457 PMID: 10098849
Heytler and Pritchard (1962) A new class of uncoupling agents - carbonyl cyanide phenylhydrazones. Biochem.Biophys.Res.Commun. 7 272 PMID: 13907155
If you know of a relevant reference for FCCP, please let us know.
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Keywords: FCCP, FCCP supplier, Oxidative, phosphorylation, uncoupler, Phosphorylation, 0453, Tocris Bioscience
12 Citations for FCCP
Citations are publications that use Tocris products. Selected citations for FCCP include:
MacDonald et al (2019) A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics. Commun Biol 2 258 PMID: 31312727
Panja et al (2019) Paraneoplastic cerebellar degeneration: Yo antibody alters mitochondrial calcium buffering capacity. Neuropathol Appl Neurobiol 45 141 PMID: 29679372
Mané and Müller (2012) Temporo-spectral imaging of intrinsic optical signals during hypoxia-induced spreading depression-like depolarization. PLoS One 7 e43981 PMID: 22952835
Janc (2014) The free radical scavenger Trolox dampens neuronal hyperexcitability, reinstates synaptic plasticity, and improves hypoxia tolerance in a mouse model of Rett syndrome. Front Cell Neurosci 8 56 PMID: 24605086
Hogan et al (2014) Divergent effects of painful nerve injury on mitochondrial Ca(2+) buffering in axotomized and adjacent sensory neurons. Brain Res 1589 112 PMID: 25251590
Burt et al (2013) 9-Phenanthrol and flufenamic acid inhibit calcium oscillations in HL-1 mouse cardiomyocytes. Cell Calcium 54 193 PMID: 23831210
Banh et al (2016) PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia. Nat Cell Biol 18 803 PMID: 27323329
Visavadiya (2016) Integrin-FAK signaling rapidly and potently promotes mitochondrial function through STAT3. Cell Communication and Signaling 14 32 PMID: 27978828
MacDonald et al (2018) Mitochondrial membrane depolarization enhances TRAIL-induced cell death in adult human granulosa tumor cells, KGN, through inhibition of BIRC5. J Ovarian Res 11 89 PMID: 30326924
Rozzi et al (2018) Human immunodeficiency virus Tat impairs mitochondrial fission in neurons. Cell Death Discov 4 8 PMID: 29531805
Gerich et al (2009) H(2)O(2)-mediated modulation of cytosolic signaling and organelle function in rat hippocampus. J Pharmacol Exp Ther 458 937 PMID: 19430810
Choubey et al (2014) BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria. Autophagy 10 1105 PMID: 24879156
Do you know of a great paper that uses FCCP from Tocris? Please let us know.
Reviews for FCCP
Average Rating: 4.5 (Based on 2 Reviews.)
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We got expected effect on cells.
By
Anonymous on 04/03/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Primary pancreatic ductal epithelial cells
4 μM FCCP
Mitochondrial bioenergetics measurements by reagent screen.
By
Anonymous on 03/15/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: mouse brain endothelioma cell line
1 μM
Literature in this Area
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Cancer Metabolism Research Product Guide
This product guide reviews some of the main areas in cancer metabolism research and lists around 150 products that can be used to investigate metabolic pathways in cancer including:
- Glycolysis
- Tricarboxylic Acid Cycle
- Lipidogenesis
- 1C Metabolism and Nucleic Acid Synthesis
- Drivers of Metabolic Reprogramming
- pH and Redox Balance