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Filipin III

Cat. No. 6250 1 Review Submit a Review Datasheet / COA / SDS
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Description: Fluorescent cholesterol stain, also polyene antibiotic. Used for: Fluorescent cholesterol stain. Binds sterol in fungal membranes. Application: confocal microscopy
Chemical Name: (3R,4S,6S,8S,10R,12R,14R,16S,17E,19E,21E,23E,25E,27S,28R)-4,6,8,10,12,14,16,27-Octahydroxy-3-[(1R)-1-hydroxyhexyl]-17,28-dimethyloxacyclooctacosa-17,19,21,23,25-pentaen-2-one
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews (1)
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Filipin III

Key information: filipin III is a fluorescent cholesterol stain; polyene antibiotic.

Used for: fluorescent cholesterol staining. Binds sterols in fungal membranes. Also inhibits formation of the pathological form of prion protein (PrP-res) from the normal membrane-bound (PrP-sen) form.

Application: confocal microscopy.

Properties and Photophysical Data: excitation and emission maxima (λ) are 405 nm and 420-480 nm, respectively.

Technical Data for Filipin III

M. Wt 654.83
Formula C35H58O11
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 480-49-9
PubChem ID 6433553
InChI Key IMQSIXYSKPIGPD-ZPXVTHDFSA-N
Smiles C[C@@H](O1)[C@@H](O)/C=C/C=C/C=C/C=C/C=C(C)/[C@@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)[C@]([C@H](O)CCCCC)([H])C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Filipin III

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 3.27 5

Preparing Stock Solutions for Filipin III

The following data is based on the product molecular weight 654.83. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 30.54 mL 152.71 mL 305.42 mL
0.25 mM 6.11 mL 30.54 mL 61.08 mL
0.5 mM 3.05 mL 15.27 mL 30.54 mL
2.5 mM 0.61 mL 3.05 mL 6.11 mL

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Product Datasheets for Filipin III

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for Filipin III

References are publications that support the biological activity of the product.

Castanho et al (1992) Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J.Biol.Chem. 267 204 PMID: 1730589

Marella et al (2002) Filipin prevents pathological prion protein accumulation by reducing endocytosis and inducing cellular PrP release. J.Biol.Chem. 277 25457 PMID: 11994310

Pipalia et al (2006) Automated microscopy screening for compounds that partially revert cholesterol accumulation in Niemann-Pick C cells. J.Lipid Res. 47 284 PMID: 16288097

If you know of a relevant reference for Filipin III, please let us know.

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Keywords: Filipin III, Filipin III supplier, polyene, antibiotics, antifungals, prions, protein, inhibitors, inhibits, PrP, fluorescent, cholesterol, stain, Antibiotics, Prion, Protein, Labels, and, Stains, Fluorescent, Cholesterol, Probes, Lipid, Cell, Membrane, Antifungals, 6250, Tocris Bioscience

Citations for Filipin III

Citations are publications that use Tocris products.

Currently there are no citations for Filipin III. Do you know of a great paper that uses Filipin III from Tocris? Please let us know.

Reviews for Filipin III

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Staining for intracellular cholesterol.
By Anonymous on 06/13/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: MEF

Fix the cells with 4% PFA for 15 min at room temperature; permeabilisation not required; incubate the cells with 0.5 mg/mL Fillipin in 3% BSA for 4 h at room temperature; mount the coverslips with DAPI-free moviol. Excite the cells with 405 nm laser, and collect signals at 410-500 nm.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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