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Submit ReviewFluorescein Tyramide
Description: Green fluorescent reagent widely used for signal amplification in IHC and FISH
Chemical Name: 3',6'-Dihydroxy-N-(4-hydroxyphenylethyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(6)-carboxamide
Purity: ≥95% (HPLC)
Biological Activity for Fluorescein Tyramide
Fluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.
Optical Data for Fluorescein Tyramide
| Emission Color | Green |
|---|---|
| λabs | 494 nm |
| λem | 517 nm |
| Extinction Coefficient (ε) | 70000 M-1cm-1 |
| Closest Laser line | 488 nm |
| Application | Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Fluorescein Tyramide
| M. Wt | 495.48 |
| Formula | C29H21NO7 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 210236-90-1 |
| InChI Key | MJQMGAFVBOOBIB-UHFFFAOYSA-N |
| Smiles | OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)C5=CC=C(C(NCCC6=CC=C(C=C6)O)=O)C=C5C(O3)=O)C=C1.OC7=CC8=C(C9(C%10=CC=C(C=C%10O8)O)C%11=CC(C(NCCC%12=CC=C(C=C%12)O)=O)=CC=C%11C(O9)=O)C=C7 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Fluorescein Tyramide
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.89 | 24 |
Preparing Stock Solutions for Fluorescein Tyramide
The following data is based on the product molecular weight 495.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.24 mM | 8.41 mL | 42.05 mL | 84.09 mL |
| 1.2 mM | 1.68 mL | 8.41 mL | 16.82 mL |
| 2.4 mM | 0.84 mL | 4.2 mL | 8.41 mL |
| 12 mM | 0.17 mL | 0.84 mL | 1.68 mL |
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Product Datasheets for Fluorescein Tyramide
References for Fluorescein Tyramide
References are publications that support the biological activity of the product.
Karkmann et al (1999) Enzymatic signal amplification for sensitive detection of intracellular antigens by flow cytometry. J.Immunol.Methods 230 113 PMID: 10594358
Schriml et al (1999) Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 27 608 PMID: 10489619
Hopman et al (1998) Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 46 771 PMID: 9603790
van Gijlswijk et al (1997) Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 45 375 PMID: 9071319
Bobrow et al (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 125 279 PMID: 2558138
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Keywords: Fluorescein Tyramide, Fluorescein Tyramide supplier, tyramide, signal, amplification, TSA, horseradish, peroxidase, HRP, green, Tyramide, Signaling, Amplification, (TSA), 6456, Tocris Bioscience
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