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Flutax 1

Cat. No. 2226 2 Citations Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells. Application: Fluorescent microscopy
Chemical Name: (2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine
Purity: ≥95% (HPLC)
Datasheet
Citations (2)
Reviews
Literature (1)
Biological Activity Scientific Data Technical Data Solubility Calculators Datasheets References

Biological Activity for Flutax 1

Key information: Flutax 1 is a green-fluorescent taxol derivative; for microtubules labeling in live cells.

Used for: direct imaging of the microtubule cytoskeleton.

Application: fluorescent microscopy.

Properties and Photophysical Data: Flutax 1 binds microtubules with high affinity (Ka ~ 107M-1). Absorption, fluorescence and fluorescence decay of Flutax 1 in solution are pH sensitive. Excitation and emission maxima (λ) are 495 nm and 520 nm, respectively.

Scientific Data

flutax 1 labeled HeLa cells View Larger

Flutax 1 labeled HeLa cells. Flutax 1 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 1 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 1 staining is not retained at all after fixation (photobleach). Staining can be done only on Live cells. Flutax 1 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good fluorescent signal.

Technical Data for Flutax 1

M. Wt 1283.3
Formula C71H66N2O21
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 191930-58-2
PubChem ID 16760423
InChI Key JMWJJMCMRRQCSY-ADONSEPTSA-N
Smiles O=C2[C@@]3(C)C([C@@]4(OC(C)=O)[C@H](OC4)C[C@@H]3OC([C@H](C)NC(C7=CC=C(C8=C(C=C%10)C(OC9=C8C=CC(O)=C9)=CC%10=O)C(C(O)=O)=C7)=O)=O)[C@H](OC(C6=CC=CC=C6)=O)[C@@]1(O)[C@](C)(C)C([C@H]2OC(C)=O)=C(C)[C@@H](OC([C@H](O)[C@H]([C@]5=CC=CC=C5)NC(C%11=CC=CC=C%11)=O)=O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Flutax 1

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 128.33 100
ethanol 128.33 100

Preparing Stock Solutions for Flutax 1

The following data is based on the product molecular weight 1283.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 0.78 mL 3.9 mL 7.79 mL
5 mM 0.16 mL 0.78 mL 1.56 mL
10 mM 0.08 mL 0.39 mL 0.78 mL
50 mM 0.02 mL 0.08 mL 0.16 mL

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Product Datasheets for Flutax 1

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for Flutax 1

References are publications that support the biological activity of the product.

Diaz et al (2000) Molecular recognition of Tax. by microtubules. Kinetics and thermodynamics of binding of fluorescent Tax. derivatives to an exposed site. J.Biol.Chem. 275 26265 PMID: 10818101

Diaz et al (2003) Fast kinetics of Tax. binding to microtubules. J.Biol.Chem. 278 8407 PMID: 12496245

Evangelio et al (1998) Fluorescent taxoids as probes of the microtubule cytoskeleton. Cell Motil.Cytoskel. 39 73 PMID: 9453715

If you know of a relevant reference for Flutax 1, please let us know.

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2 Citations for Flutax 1

Citations are publications that use Tocris products. Selected citations for Flutax 1 include:

Kaittanis et al (2015) Multifunctional MRI/PET Nanobeacons Derived from the in Situ Self-Assembly of Translational Polymers and Clinical Cargo through Coalescent Intermolecular Forces. Nano Lett 15 8032 PMID: 26540670

Kaittanis et al (2014) Environment-responsive nanophores for therapy and treatment monitoring via molecular MRI quenching. Nat Commun 5 3384 PMID: 24594970

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Literature in this Area

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