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Submit ReviewFlutax 2
Description: Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions
Chemical Name: (2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(2',7'-difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine
Purity: ≥90% (HPLC)
Biological Activity for Flutax 2
Key information: Flutax 2 is a green-fluorescent taxol derivative; for microtubules labeling in live cells.
Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions.
Application: fluorescence microscopy.
Properties and Photophysical Data: The fluorescent label on Flutax 2 is attached by derivatization of the 7-β-hydroxy group of native paclitaxel which permits selective binding of the probe to microtubules. Flutax 2 binds microtubules with high affinity (Ka ~ 107M-1). Flutax 2 binds to α, β tubulin dimers and stabilizes them. Flutax 2 is soluble and pH insensitive and is more photostable than Flutax 1 (Cat. No. 2226). Excitation and emission maxima (λ) are 496 nm and 526 nm, respectively.
Scientific Data
Fluorescence Imaging
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Flutax 2 labeled HeLa cells. Flutax 2 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 2 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 2 staining is not retained at all after fixation (photobleach). Like Flutax 1, Flutax 2 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good signal.
Optical Data for Flutax 2
| λabs | 496 nm |
|---|---|
| λem | 526 nm |
| Extinction Coefficient (ε) | 80000 M-1cm-1 |
| Quantum Yield (φ) | 0.9 |
| Application | Fluorescence Microscopy |
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Technical Data for Flutax 2
| M. Wt | 1319.28 |
| Formula | C71H64F2N2O21 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 301844-13-3 |
| PubChem ID | 44571006 |
| InChI Key | UTOYCOKRJWZPNT-SQNCZLKBSA-N |
| Smiles | O=C1[C@@]2(C)C([C@@]3(OC(C)=O)[C@H](OC3)C[C@@H]2OC([C@H](C)NC(C4=CC=C(C5=C(C=C6F)C(OC7=C5C=C(F)C(O)=C7)=CC6=O)C(C(O)=O)=C4)=O)=O)[C@H](OC(C8=CC=CC=C8)=O)[C@@]9(O)C(C)(C)C([C@H]1OC(C)=O)=C(C)[C@@H](OC([C@H](O)[C@H](C%10=CC=CC=C%10)NC(C%11=CC=CC=C%11)=O)=O)C9 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Flutax 2
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 1.32 | 1 |
Preparing Stock Solutions for Flutax 2
The following data is based on the product molecular weight 1319.28. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.01 mM | 75.8 mL | 378.99 mL | 757.99 mL |
| 0.05 mM | 15.16 mL | 75.8 mL | 151.6 mL |
| 0.1 mM | 7.58 mL | 37.9 mL | 75.8 mL |
| 0.5 mM | 1.52 mL | 7.58 mL | 15.16 mL |
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Product Datasheets for Flutax 2
References for Flutax 2
References are publications that support the biological activity of the product.
Lillo et al (2002) Location and properties of the Tax. binding center in microtubules: a picosecond laser study with fluorescent taxoids. Biochemistry. 41 12436 PMID: 12369834
Diaz et al (2000) Molecular recognition of Tax. by microtubules. Kinetics and thermodynamics of binding of fluorescent Tax. derivatives to an exposed site. J.Biol.Chem. 275 26265 PMID: 10818101
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Keywords: Flutax 2, Flutax 2 supplier, paclitaxel, Flutax2, Taxol, fluorescent, dye, labeled, labelled, microtubules, tubulin, oregon, green, Microtubules, Mitosis, Organelle, and, Cell, Structure, Stains, Microtubule, Probes, 6254, Tocris Bioscience
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