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Description: Fluorescent Ca2+ indicator
Chemical Name: 2-[6-[bis[2-[(Acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl ester
Purity: ≥95% (HPLC)
Biological Activity for FURA-2AM
FURA-2AM is a fluorescent ratiometric Ca2+ indicator (Kd Ca2+ = 145 nM). FURA-2AM is selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. FURA-2AM binds to free intracellular calcium and is used to determine [Ca2+]i concentration.
This product is typically prepared in DMSO. F 127 (Cat. No. 6253) for the solubilization of FURA-2AM is also available.
Optical Data for FURA-2AM
| λabs | 371 nm |
|---|---|
| λem | 474 nm |
| Extinction Coefficient (ε) | 30000 M-1cm-1 |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Fluorescent Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for FURA-2AM
| M. Wt | 1001.85 |
| Formula | C44H47N3O24 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 108964-32-5 |
| PubChem ID | 90488749 |
| InChI Key | JTDRWQMCMPIBNJ-UHFFFAOYSA-N |
| Smiles | CC1=CC(OCCOC2=C(N(CC(OCOC(C)=O)=O)CC(OCOC=O)=O)C=C(OC(C4=NC=C(C(OCOC(C)=O)=O)O4)=C3)C3=C2)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for FURA-2AM
References for FURA-2AM
References are publications that support the biological activity of the product.
Grynkiewicz et al (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J.Biol.Chem. 260 3440 PMID: 3838314
Wang et al (2008) Sildenafil inhibits human pulmonary artery smooth muscle cell proliferation by decreasing capacitative Ca2+ entry. J.Pharmacol.Sci. 108 71 PMID: 18818482
If you know of a relevant reference for FURA-2AM, please let us know.
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Keywords: FURA-2AM, FURA-2AM supplier, Fluorescent, Ca2+, indicator, Probes, Calcium, Signaling, Signalling, Agents, General, ratiometric, Ion, Indicators, 2220, Tocris Bioscience
3 Citations for FURA-2AM
Citations are publications that use Tocris products. Selected citations for FURA-2AM include:
Bayliss and Evans (2013) Characterisation of AmphiAmR11, an amphioxus (Branchiostoma floridae) D2-DA-like G protein-coupled receptor. PLoS One 8 e80833 PMID: 24265838
Cabrita et al (2019) Niclosamide repurposed for the treatment of inflammatory airway disease. JCI Insight 4 PMID: 31391337
Luo et al (2015) KA attenuates the Na+-dependent Ca2+ overload in rabbit ventricular myocytes in vitro by inhibiting late Na+ and L-type Ca2+ currents. Mol Neurodegener 36 1327 PMID: 26456586
Do you know of a great paper that uses FURA-2AM from Tocris? Please let us know.
Reviews for FURA-2AM
Average Rating: 4.7 (Based on 3 Reviews.)
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Detects store-operated Ca2+ entry after ER store depletion in RBL-2H3 cells..
By
Niroj Shrestha on 11/02/2018
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: RBL-2H3
RBL-2H3 cells at a confluency of 50–60% were cultured on glass coverslip and loaded with 2 μM Fura-2AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. The Fura-2 loaded cells were excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm every 1.2 s. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.
FURA-2AM as a tool to analyse calcium transient in cardiomyocytes.
By
Christopher Trummer on 10/15/2018
Assay Type: In Vitro
Species: Other
Cell Line/Tissue: primary ventricular cardiomyocytes
Primary guinea pig ventricular cardiomyocytes were loaded with FURA-2AM (1 μM) and Pluronic F 127 (1%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured.
Detects cytosolic calcium influx changes well.
By
Anonymous on 11/01/2017
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: spinal cord neurons
Used in live cell calcium imaging assay to study glutamate receptor signaling in neurons. Used at a concentration of 5 micromolar (along with pluronic). Images shows changes in fura-2 fluorescence pre (left) and post(right) glutamate stimulation.
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