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Submit ReviewHoechst 33258
Description: Fluorescent dye for staining DNA
Chemical Name: 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
Purity: ≥98% (HPLC)
Biological Activity for Hoechst 33258
Hoechst 33258 is a fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.
Optical Data for Hoechst 33258
| λabs | 352 nm |
|---|---|
| λem | 461 nm |
| Application | Flow Cytometry |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Hoechst 33258
| M. Wt | 533.88 |
| Formula | C25H24N6O.3HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 23491-45-4 |
| PubChem ID | 5360743 |
| InChI Key | OKQAASMPIOSOEX-UHFFFAOYSA-N |
| Smiles | CN(CC1)CCN1C2=CC(N=C(C3=CC(NC(C4=CC=C(O)C=C4)=N5)=C5C=C3)N6)=C6C=C2.Cl.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Hoechst 33258
References for Hoechst 33258
References are publications that support the biological activity of the product.
Oberhammer et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12 3679 PMID: 8253089
Jin and Breslauer (1988) Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Proc.Natl.Acad.Sci.U.S.A. 85 8939 PMID: 2461559
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Keywords: Hoechst 33258, Hoechst 33258 supplier, DNA, minor, groove, binds, fluorescent, dyes, probes, Fluorescent, Stains, Cell, Viability, and, Dyes, Counterstains, Flow, Cytometry, 5824, Tocris Bioscience
2 Citations for Hoechst 33258
Citations are publications that use Tocris products. Selected citations for Hoechst 33258 include:
Terheyden-Keighley et al (2018) CXCR4/SDF1 signalling promotes sensory neuron clustering in vitro. Biol Open 7 PMID: 30135081
Moreno et al (2019) Mitotic replisome disassembly depends on TRAIP ubiquitin ligase activity. Life Sci Alliance 2 PMID: 30979826
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals