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Submit ReviewHoechst Janelia Fluor® 526
Description: Fluorogenic, green-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, flow cytometry, super resolution microscopy (SRM)
Alternative Names: JF526-HST
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-((2-(2-(2-(4-(4-(6-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate tetratrifluoroacetate
Purity: ≥95% (HPLC)
Biological Activity for Hoechst Janelia Fluor® 526
Key information: Hoechst Janelia Fluor® 526 is a fluorogenic, green-emitting DNA probe. Suitable for live cell imaging.
Used for: fixed and live-cell imaging.
Application: confocal microscopy, flow cytometry, super resolution microscopy (SRM).
Properties and Photophysical Data: preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor® 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor® 526 exhibits a quantum yield of 0.04. Fluorogenic, it fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 526 can be combined with fluorogenic red-emitting DNA probe Hoechst Janelia Fluor® 646 (Cat. No. 6804) for multiplexing experiments. Excitation and emission maxima (λ) are 531 nm and 549 nm, respectively; quantum yield = 0.126; extinction coefficient = 118,000 M-1cm-1.
To measure the absorbance spectrum of Hoechst Janelia Fluor® 526 we recommend the following solvent: TFE plus 0.1% TFA.
Scientific Data
Confocal Microscopy
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Application of Hoechst Janelia Fluor® 526 in U2OS cells. Fixed U2OS cells were stained with Hoechst Janelia Fluor® 526 (2 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Hoechst Janelia Fluor® 526
| Emission Color | Yellow |
|---|---|
| λabs | 531 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 118,000 M-1cm-1 |
| Quantum Yield (φ) | 0.126 |
| Application | Confocal Microscopy, Flow Cytometry, Super Resolution Microscopy (SRM) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Hoechst Janelia Fluor® 526
| M. Wt | 1641.27 |
| Formula | C62H58F6N10O8.4CF3CO2H |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | AFTKOWWBAAQRNW-UHFFFAOYSA-N |
| Smiles | CN1CCN(CC1)C2=CC=C3N=C(NC3=C2)C4=CC=C5N=C(NC5=C4)C6=CC=C(OCCCC(NCCOCCOCCNC(C7=CC(C8=C9C=C(F)/C(C=C9OC%10=C8C=C(F)C(N%11CC(C%11)(F)F)=C%10)=[N+]%12CC(C\%12)(F)F)=C(C=C7)C([O-])=O)=O)=O)C=C6.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Hoechst Janelia Fluor® 526
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 12.99 | 10 |
Preparing Stock Solutions for Hoechst Janelia Fluor® 526
The following data is based on the product molecular weight 1641.27. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 6.09 mL | 30.46 mL | 60.93 mL |
| 0.5 mM | 1.22 mL | 6.09 mL | 12.19 mL |
| 1 mM | 0.61 mL | 3.05 mL | 6.09 mL |
| 5 mM | 0.12 mL | 0.61 mL | 1.22 mL |
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Product Datasheets for Hoechst Janelia Fluor® 526
References for Hoechst Janelia Fluor® 526
References are publications that support the biological activity of the product.
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for Hoechst Janelia Fluor® 526, please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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