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Submit ReviewJanelia Fluor® 549, Azide
Discontinued Product
6501 has been discontinued.
Description: Yellow dye (Reactive group: azide). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging
Chemical Name: 3,6-Di-1-azetidinyl-9-[5-[[2-[2-[2-[2-azidoethoxy]ethoxy]ethoxy]ethyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
Purity: ≥90% (HPLC)
Biological Activity for Janelia Fluor® 549, Azide
Janelia Fluor® 549, Azide is a fluorescent yellow dye; supplied as an azide for click chemisty. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 549, Azide
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Azide |
| Reactivity | Click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal imaging, dSTORM, STED, Flow |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Janelia Fluor® 549, Azide
| M. Wt | 654.71 |
| Formula | C35H38N6O7 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| PubChem ID | 137919860 |
| InChI Key | XDSXVSGQYCACTI-UHFFFAOYSA-N |
| Smiles | O=C(NCCOCCOCCOCCN=[N+]=[N-])C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CCC3)C=C2O4)=C(C=C/5)C4=CC5=[N+]6CCC\6)=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Janelia Fluor® 549, Azide
References for Janelia Fluor® 549, Azide
References are publications that support the biological activity of the product.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551
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Citations for Janelia Fluor® 549, Azide
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Currently there are no citations for Janelia Fluor® 549, Azide.
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Fluorescent Dyes and Probes Research Product Guide
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