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Submit ReviewJanelia Fluor® 549, Tetrazine
Description: Yellow dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Chemical Name: 3,6-Di-1-azetidinyl-9-[[4-[(1,2,4,5-tetrazin-3-yl)benzyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
Biological Activity for Janelia Fluor® 549, Tetrazine
Key Information: Janelia Fluor® 549, Tetrazine is a yellow fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live-cell imaging.
Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Janelia Fluor® 549, tetrazine is cell permeable. It can be coupled directly to a protein of interest using bioorthogonal chemistry and unnatural amino acid technology and has been used in this way to label living primary neurons.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 549, Tetrazine
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Tetrazine |
| Reactivity | Copper-free click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal Microscopy, dSTORM, STED, Live-cell Imaging |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data for Janelia Fluor® 549, Tetrazine
| M. Wt | 623.66 |
| Formula | C36H29N7O4 |
| Storage | Store at -20°C |
| Smiles | O=C(NCC1=CC=C(C2=NN=CN=N2)C=C1)C3=CC=C(C([O-])=O)C(C(C4=CC=C(N5CCC5)C=C4O6)=C(C=C/7)C6=CC7=[N+]8CCC\8)=C3 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 549, Tetrazine
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.24 | 10 |
Preparing Stock Solutions for Janelia Fluor® 549, Tetrazine
The following data is based on the product molecular weight 623.66. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 16.03 mL | 80.17 mL | 160.34 mL |
| 0.5 mM | 3.21 mL | 16.03 mL | 32.07 mL |
| 1 mM | 1.6 mL | 8.02 mL | 16.03 mL |
| 5 mM | 0.32 mL | 1.6 mL | 3.21 mL |
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Product Datasheets for Janelia Fluor® 549, Tetrazine
References for Janelia Fluor® 549, Tetrazine
References are publications that support the biological activity of the product.
Arsic et al (2022) Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Nat.Commun. 13 PMID: 35031604
If you know of a relevant reference for Janelia Fluor® 549, Tetrazine, please let us know.
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2 Citations for Janelia Fluor® 549, Tetrazine
Citations are publications that use Tocris products. Selected citations for Janelia Fluor® 549, Tetrazine include:
Yu et al (2023) Visualizing the disordered nuclear transport machinery in situ. Nature 617 162 PMID: 37100914
Arsic et al (2022) Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Nat.Commun. 13 PMID: 35031604
Do you know of a great paper that uses Janelia Fluor® 549, Tetrazine from Tocris? Please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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