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Submit ReviewJanelia Fluor® 635, Tetrazine New
Description: Red dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Chemical Name: 4-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥90% (HPLC)
Biological Activity for Janelia Fluor® 635, Tetrazine
Key Information: Janelia Fluor® 635, Tetrazine is a red fluorogenic fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live-cell imaging.
Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Janelia Fluor® 635, Tetrazine is cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 645 nm and 663 nm, respectively.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 635, Tetrazine
| Emission Color | Red |
|---|---|
| λabs | 635 nm |
| λem | 652 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry |
| Alternative For | Alexa Fluor® 633, Atto 633, CF633 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data for Janelia Fluor® 635, Tetrazine
| M. Wt | 701.81 |
| Formula | C38H33F2N7O3Si |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| InChI Key | DVDISOMTYSQEME-UHFFFAOYSA-N |
| Smiles | C[Si](C1=C/C2=[N+]3CC(C/3)F)(C4=CC(N5CC(C5)F)=CC=C4C(C6=CC(C(NCC7=CC=C(C8=NN=CN=N8)C=C7)=O)=CC=C6C([O-])=O)=C1C=C2)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 635, Tetrazine
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.02 | 10 |
Preparing Stock Solutions for Janelia Fluor® 635, Tetrazine
The following data is based on the product molecular weight 701.81. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 14.25 mL | 71.24 mL | 142.49 mL |
| 0.5 mM | 2.85 mL | 14.25 mL | 28.5 mL |
| 1 mM | 1.42 mL | 7.12 mL | 14.25 mL |
| 5 mM | 0.28 mL | 1.42 mL | 2.85 mL |
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Product Datasheets for Janelia Fluor® 635, Tetrazine
References for Janelia Fluor® 635, Tetrazine
References are publications that support the biological activity of the product.
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Fluorescent Dyes and Probes Research Product Guide
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