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Submit ReviewMinocycline hydrochloride
Description: Antibiotic. Displays neuroprotective effects
Chemical Name: [4S-(4α,4aα,5aα,12aα)]-4,7-Bis(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,10,12,12a,tetrahydroxy-1,11-dioxo-2-naphthacenecarboxamide
Purity: ≥98% (HPLC)
Biological Activity for Minocycline hydrochloride
Minocycline hydrochloride is a tetracycline antibiotic. Displays neuroprotective, antiapoptotic, anti-inflammatory and antimicrobial effects. Acts as a matrix metalloproteinase (MMP) inhibitor; attenuates disease severity in mouse models of multiple sclerosis. Orally active and brain penetrant. Allows formation of extended pluripotent stem (EPS) cells in combination with CHIR 99021 (Cat.No. 4423), (S)-(+)-Dimethindene maleate (Cat.No. 1425) and human leukemia inhibitory factor.
For more information about how Minocycline hydrochloride may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).
Technical Data for Minocycline hydrochloride
| M. Wt | 493.94 |
| Formula | C23H27N3O7.HCl |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 13614-98-7 |
| PubChem ID | 54685925 |
| InChI Key | WTJXVDPDEQKTCV-VQAITOIOSA-N |
| Smiles | NC(C4=C(O)[C@@H](N(C)C)[C@@]3([H])[C@](C4=O)(O)C(O)=C2C(C1=C(O)C=CC(N(C)C)=C1C[C@]([H])2C3)=O)=O.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Minocycline hydrochloride
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 49.39 | 100 | |
| water | 12.35 | 25 |
Preparing Stock Solutions for Minocycline hydrochloride
The following data is based on the product molecular weight 493.94. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.02 mL | 10.12 mL | 20.25 mL |
| 5 mM | 0.4 mL | 2.02 mL | 4.05 mL |
| 10 mM | 0.2 mL | 1.01 mL | 2.02 mL |
| 50 mM | 0.04 mL | 0.2 mL | 0.4 mL |
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Product Datasheets for Minocycline hydrochloride
References for Minocycline hydrochloride
References are publications that support the biological activity of the product.
Tikka et al (2001) MinCyc, a tetracy. derivative, is neuroprotective against excitotoxicity by inhibiting activation and proliferation of microglia. J.Neurosci. 21 2580 PMID: 11306611
Brundula et al (2002) Targeting leukocyte MMPs and transmigration: MinCyc as a potential therapy for multiple sclerosis. Brain 125 1297 PMID: 12023318
Padi and Kulkarni (2008) MinCyc prevents the development of neuropathic pain, but not acute pain: possible anti-inflammatory and antioxidant mechanisms. Eur.J.Pharmacol. 601 79 PMID: 18952075
Yang et al (2017) Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency. Cell. 169 243 PMID: 28388409
If you know of a relevant reference for Minocycline hydrochloride, please let us know.
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Keywords: Minocycline hydrochloride, Minocycline hydrochloride supplier, Minocycline, HCl, antibiotics, MMP, matrix, metalloproteinase, inhibitors, inhibits, neuroprotective, Antibiotics, Matrix, Metalloprotease, Stem, Cell, Reprogramming, 3268, Tocris Bioscience
⚠ WARNING: This product can expose you to chemicals including Minocycline hydrochloride (internal use), which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov
2 Citations for Minocycline hydrochloride
Citations are publications that use Tocris products. Selected citations for Minocycline hydrochloride include:
Yaeger and Trussell (2015) Single granule cells excite Golgi cells and evoke feedback inhibition in the cochlear nucleus. J Neurosci 35 4741 PMID: 25788690
Song et al (2015) MinCyc does not affect long-term potentiation in the anterior cingulate cortex of normal adult mice. Hum Mol Genet 11 25 PMID: 25933605
Do you know of a great paper that uses Minocycline hydrochloride from Tocris? Please let us know.
Reviews for Minocycline hydrochloride
Average Rating: 5 (Based on 1 Review.)
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Blocker of microglia reactivity.
By
Luiz Alexandre Magno on 07/16/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Brain
I have used the antibiotic minocycline to impair activation of microglia in the brain of mice. This drug has anti-inflammatory properties that block microglial reactivity, which can be easily confirmed by expression of the CD68 protein. The drug was diluted in PBS and injected i.p. (75 mg/kg) in mice, twice a day, for 7 days. The image shows that microglial cells (stained for Iba1) do not express CD68 (in red) after treatment with minocycline.
