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Submit ReviewPhen Green SK diacetate
Description: Heavy metal ion indicator, membrane permeable
Chemical Name: 3',6'-Bis(acetyloxy)-2',7'-dichloro-3-oxo-N-1,10-phenanthrolin-5-yl-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxamide
Purity: ≥90% (HPLC)
Biological Activity for Phen Green SK diacetate
Phen Green SK diacetate is a fluorescent heavy metal ion indicator that can be used to detect various metal ions including Cu2+, Cu+, Fe2+, Hg2+, Pb2+, Cd2+, Zn2+ and Ni2+. Excitation and emission maxima (λ) are 507 and 532 nm, respectively. Intracellular Phen Green SK diacetate fluorescence is quenched upon reaction with metal ions. This product is cell permeable.
It is recommended to prepare stock solutions in DMSO.
Optical Data for Phen Green SK diacetate
| λabs | 507 nm |
|---|---|
| λem | 532 nm |
| Extinction Coefficient (ε) | 40000 M-1cm-1 |
| Cell Permeable | Yes |
| Application | Fluorescence Microscopy, Flow Cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Phen Green SK diacetate
| M. Wt | 706.49 |
| Formula | C37H21Cl2N3O8 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 234075-45-7 |
| PubChem ID | 137700453 |
| InChI Key | INOXUAMBIQQWOK-UHFFFAOYSA-N |
| Smiles | O=C1OC2(C3=CC=C(C(NC4=CC5=CC=CN=C5C6=C4C=CC=N6)=O)C=C13)C7=CC(Cl)=C(C=C7OC8=CC(OC(C)=O)=C(C=C82)Cl)OC(C)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Phen Green SK diacetate
References for Phen Green SK diacetate
References are publications that support the biological activity of the product.
Shen et al (2022) Geniposide possesses the protective effect on myocardial injury by inhibiting oxidative stress and ferroptosis via activation of the Grsf1/GPx4 Axis. Front.Pharmacol. 13 879870 PMID: 35600863
Kim et al (2006) Calibration of Phen Green for use as a Cu(I)-selective fluorescent indicator. Anal.Chim.Acta 575 223 PMID: 17723595
Lu et al (2022) Epigenetic regulation of ferroptosis via ETS1/miR-23a-3p/ACSL4 axis mediates sorafenib resistance in human hepatocellular carcinoma. J.Exp.Clin.Cancer Res. 41 3 PMID: 34980204
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Fluorescent Dyes and Probes Research Product Guide
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