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(S)-(-)-Blebbistatin

Cat. No. 1852 3 Citations Submit a Review Datasheet / COA / SDS
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Description: Selective inhibitor of myosin II ATPase activity. Active enantiomer of (±)-blebbistatin (Cat. No. 1760)
Chemical Name: (3aS)-(-)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one
Purity: ≥99% (HPLC)
Datasheet
Citations (3)
Reviews
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for (S)-(-)-Blebbistatin

(S)-(-)-Blebbistatin is a selective inhibitor of myosin II ATPase activity (IC50 ~0.5 - 5 μM); active enantiomer of (±)-blebbistatin. Inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. (S)-(-)-Blebbistatin rapidly and reversibly blocks cell blebbing, and disrupts directed cell migration and cytokinesis in vertebrate cells.

Inactive Enantiomer and Racemate also available.

Technical Data for (S)-(-)-Blebbistatin

M. Wt 292.34
Formula C18H16N2O2
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 856925-71-8
PubChem ID 5287792
InChI Key LZAXPYOBKSJSEX-GOSISDBHSA-N
Smiles O=C2[C@@]3(O)C(N(C4=CC=CC=C4)CC3)=NC1=CC=C(C)C=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for (S)-(-)-Blebbistatin

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 29.23 100

Preparing Stock Solutions for (S)-(-)-Blebbistatin

The following data is based on the product molecular weight 292.34. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.42 mL 17.1 mL 34.21 mL
5 mM 0.68 mL 3.42 mL 6.84 mL
10 mM 0.34 mL 1.71 mL 3.42 mL
50 mM 0.07 mL 0.34 mL 0.68 mL

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Product Datasheets for (S)-(-)-Blebbistatin

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for (S)-(-)-Blebbistatin

References are publications that support the biological activity of the product.

Limouze et al (2004) Specificity of blebbistatin, an inhibitor of myosin II. J.Muscle Res.Cell Motil. 25 337 PMID: 15548862

Straight et al (2003) Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 299 1743 PMID: 12637748

Straight et al (2002) Dissecting cytokinesis with small molecules. Amer.Soc.Cell Biol.Abstr. 2515

If you know of a relevant reference for (S)-(-)-Blebbistatin, please let us know.

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Keywords: (S)-(-)-Blebbistatin, (S)-(-)-Blebbistatin supplier, Selective, inhibitors, inhibits, myosin, II, ATPase, Myosin, 1852, Tocris Bioscience

3 Citations for (S)-(-)-Blebbistatin

Citations are publications that use Tocris products. Selected citations for (S)-(-)-Blebbistatin include:

Bzymek et al (2016) Real-time two- and three-dimensional imaging of monocyte motility and navigation on planar surfaces and in collagen matrices: roles of Rho. Sci Rep 6 25016 PMID: 27122054

Maître et al (2016) Asymmetric division of contractile domains couples cell positioning and fate specification. Nature 536 344 PMID: 27487217

Schiff et al (2019) P38 inhibition reverses TGFβ1 and TNFα-induced contraction in a model of proliferative vitreoretinopathy. Commun Biol 2 162 PMID: 31069271

Do you know of a great paper that uses (S)-(-)-Blebbistatin from Tocris? Please let us know.

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