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sCy5DL-amide

Cat. No. 7835 Submit a Review Datasheet / COA / SDS
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Description: FDAA for super-resolution microscopy of bacteria
Chemical Name: 2-((1E,3E)-5-((E)-1-(6-(((R)-6-Amino-5-ammonio-6-oxohexyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)penta-1,3-dien-1-yl)-1,3,3-trimethyl-3H-indol-1-ium-5-sulfonate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Scientific Data Technical Data Solubility Calculators Datasheets References

Biological Activity for sCy5DL-amide

sCy5DL-amide is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into Gram-positive B.subtilis. sCy5DL-amide labeled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/666 nm.

Scientific Data

Fluorescence Imaging Bacillus subtilis cells stained with sCy5DA-amide View Larger

Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA-amide

Optical Data for sCy5DL-amide

sCy5DL-amide Dye Spectra
Emission Color Red
λabs 646 nm
λem 666 nm
Extinction Coefficient (ε) 209,200 M-1cm-1
Cell Permeable Yes
Application STORM

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for sCy5DL-amide

M. Wt 769.97
Formula C38H51N5O8S2
Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key KASKHZOOXRBMET-SSEXGKCCSA-N
Smiles CC1(C2=C(N(/C1=C/C=C/C=C/C3=[N+](C)C4=C(C=C(C=C4)S(=O)([O-])=O)C3(C)C)CCCCCC(NCCCC[C@@H]([NH3+])C(N)=O)=O)C=CC(S(=O)([O-])=O)=C2)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for sCy5DL-amide

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.7 10

Preparing Stock Solutions for sCy5DL-amide

The following data is based on the product molecular weight 769.97. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 12.99 mL 64.94 mL 129.88 mL
0.5 mM 2.6 mL 12.99 mL 25.98 mL
1 mM 1.3 mL 6.49 mL 12.99 mL
5 mM 0.26 mL 1.3 mL 2.6 mL

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References for sCy5DL-amide

References are publications that support the biological activity of the product.

Zhang et al (2022) Fluorescent D-amino acids for super-resolution microscopy of the bacterial cell wall. ACS Chem.Biol. 117 2418 PMID: 35994360

If you know of a relevant reference for sCy5DL-amide, please let us know.

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Citations for sCy5DL-amide

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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