Spautin 1

Pricing Availability   Qty
Description: USP10 and USP13 inhibitor; inhibits autophagy
Chemical Name: 6-Fluoro-N-[(4-Fluorophenyl)methyl]-4-quinazolinamine
Purity: ≥98% (HPLC)
Datasheet
Citations (3)
Reviews

Biological Activity for Spautin 1

Spautin 1 is an inhibitor of autophagy; inhibits USP10 and USP13 activity (IC50 values are 0.6 and 0.7 μM respectively) and promotes degradation of Vps34 (class III PI 3-kinase) complexes. Selectively promotes apoptosis of cancer cells under starvation conditions. Prevents the PDGF-induced conversion from the contractile to synthetic phenotype in vascular smooth muscle cells.

Compound Libraries for Spautin 1

Spautin 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Spautin 1

M. Wt 271.26
Formula C15H11F2N3
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1262888-28-7
PubChem ID 51037431
InChI Key AWIVHRPYFSSVOG-UHFFFAOYSA-N
Smiles FC1=CC=C(N=CN=C2NCC3=CC=C(F)C=C3)C2=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Spautin 1

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 27.13 100

Preparing Stock Solutions for Spautin 1

The following data is based on the product molecular weight 271.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.69 mL 18.43 mL 36.87 mL
5 mM 0.74 mL 3.69 mL 7.37 mL
10 mM 0.37 mL 1.84 mL 3.69 mL
50 mM 0.07 mL 0.37 mL 0.74 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for Spautin 1

References are publications that support the biological activity of the product.

Salabei et al (2013) PDGF-mediated autophagy regulates vascular smooth muscle cell phenotype and resistance to oxidative stress. Biochem.J. 451 375 PMID: 23421427

Liu et al (2011) Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13. Cell 147 223 PMID: 21962518


If you know of a relevant reference for Spautin 1, please let us know.

View Related Products by Product Action

View all Deubiquitinating Enzyme Inhibitors

Keywords: Spautin 1, Spautin 1 supplier, class, III, PI3, kinase, autophagy, inhibitors, inhibits, USP10, USP13, deubiquitinating, enzymes, vps34, Deubiquitinating, Enzymes, Autophagy, 5197, Tocris Bioscience

3 Citations for Spautin 1

Citations are publications that use Tocris products. Selected citations for Spautin 1 include:

Tinghu et al (2021) Targeting oncoproteins with a positive selection assay for protein degraders. Sci Adv 7 PMID: 33547076

Ji Eun et al (2022) USP13 regulates HMGB1 stability and secretion through its deubiquitinase activity. Mol Med 28 164 PMID: 36585612

Catarina et al (2020) Antagonistic relationship between the unfolded protein response and myocardin-driven transcription in smooth muscle. J Cell Physiol 235 7370-7382 PMID: 32039481


Do you know of a great paper that uses Spautin 1 from Tocris? Please let us know.

Reviews for Spautin 1

There are currently no reviews for this product. Be the first to review Spautin 1 and earn rewards!

Have you used Spautin 1?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review