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Submit ReviewEGCG
Description: β-secretase (BACE) inhibitor; inhibits amyloid assembly
Alternative Names: Epigallocatechin gallate
Chemical Name: 3,4,5-Trihydroxybenzoic acid (2R,3R)-3,4-dihydro-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-2H-1-benzopyran-3-yl ester
Purity: ≥98% (HPLC)
Biological Activity for EGCG
EGCG is a β-secretase (BACE1) inhibitor (IC50 = 1.6 μM); inhibits amyloid assembly. Inhibits the formation of amyloid fibrils by proteins such as Aβ, α-synuclein and huntingtin. Reduces Aβ42-induced cell death in three different cell types. Also inhibits glutamate dehydrogenase. Inhibits cell growth and selectively induces apoptosis in A431 and human pancreatic cancer cells. Also a DNMT1 inhibitor.
Technical Data for EGCG
| M. Wt | 458.37 |
| Formula | C22H18O11 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 989-51-5 |
| PubChem ID | 65064 |
| InChI Key | WMBWREPUVVBILR-WIYYLYMNSA-N |
| Smiles | O=C(C4=CC(O)=C(O)C(O)=C4)O[C@@H]2CC1=C(O)C=C(O)C=C1O[C@@H]2[C@]3=CC(O)=C(O)C(O)=C3 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for EGCG
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 4.58 | 10 | |
| DMSO | 45.84 | 100 |
Preparing Stock Solutions for EGCG
The following data is based on the product molecular weight 458.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.18 mL | 10.91 mL | 21.82 mL |
| 5 mM | 0.44 mL | 2.18 mL | 4.36 mL |
| 10 mM | 0.22 mL | 1.09 mL | 2.18 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.44 mL |
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Product Datasheets for EGCG
References for EGCG
References are publications that support the biological activity of the product.
Jeon et al (2003) Green tea catechins as a BACE1 (β-secretase) inhibitor. Bioorg.Med.Chem.Lett. 13 3905 PMID: 14592472
Sinha et al (2012) Comparison of three amyloid assembly inhibitors: the sugar scyllo-inositol, the polyphenol epigallocatechin gallate, and the molecular tweezer CLR01. ACS Chem.Neurosci. 3 451 PMID: 22860214
Ahmad et al (2000) Green tea polyphenol epigallocatechin-3-gallate differentially modulates nuclear factor κB in cancer cells versus normal cells. Ach.Biochem.Biophys. 376 338 PMID: 10775421
Shankar et al (2008) EGCG inhibits growth, invasion, angiogenesis and metastastasis of pancreatic cancer. Front.Biosci. 13 440 PMID: 17981559
Lee et al (2005) Mechanisms for the inhibition of DNA methyltransferases by tea catechins and bioflavonoids. Mol.Pharmacol. 68 1018 PMID: 16037419
Yang et al (2009) Glioblastoma cells require glutamate dehydrogenase to survive impairments of glucose metabolism or Akt signaling. Cancer Res. 69 7986 PMID: 19826036
If you know of a relevant reference for EGCG, please let us know.
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Keywords: EGCG, EGCG supplier, BACE1, inhibitors, inhibits, β-secretase, b-secretase, beta-secretase, flavonoids, green, tea, antioxidants, neuroprotective, EGCG, epigallocatechin, gallate, amyloid, assembly, amyloidbeta, amyloidb, amyloidβ, glutamate, dehydrogenase, GDH, DNMT, DNA, Methyltransferas, Epigallocatechin, Amyloid, Beta, Peptides, Methyltransferases, Other, Proteases, 4524, Tocris Bioscience
11 Citations for EGCG
Citations are publications that use Tocris products. Selected citations for EGCG include:
Rainer et al (2020) The Aggregation Conditions Define Whether EGCG is an Inhibitor or Enhancer of α-Synuclein Amyloid Fibril Formation. Int J Mol Sci 21 PMID: 32183378
C et al (2020) The green tea polyphenol epigallocatechin-3-gallate (EGCG) restores CDKL5-dependent synaptic defects in vitro and in vivo. Neurobiol Dis 138 104791 PMID: 32032735
Zhefeng et al (2020) Distinguishing the Effect on the Rate and Yield of Aβ42 Aggregation by Green Tea Polyphenol EGCG. ACS Omega 5 21497-21505 PMID: 32905372
Daniels et al (2019) Cyclized NDGA modifies dynamic α-synuclein monomers preventing aggregation and toxicity. Sci Rep 9 2937 PMID: 30814575
Xue et al (2019) Aβ42 fibril formation from predominantly oligomeric samples suggests a link between oligomer heterogeneity and fibril polymorphism. R Soc Open Sci 6 190179 PMID: 31417723
Michael F et al (2021) EGCG Inhibits Tumor Growth in Melanoma by Targeting JAK-STAT Signaling and Its Downstream PD-L1/PD-L2-PD1 Axis in Tumors and Enhancing Cytotoxic T-Cell Responses. Pharmaceuticals (Basel) 14 PMID: 34832863
Wei et al (2019) Epigallocatechin-3-Gallate (EGCG) Suppresses Pancreatic Cancer Cell Growth, Invasion, and Migration partly through the Inhibition of Akt Pathway and Epithelial-Mesenchymal Transition: Enhanced Efficacy when Combined with Gemcitabine. Nutrients 11 PMID: 31405071
Warren et al (2022) Evaluation of Glutaminolysis in T Cells. Curr Protoc 2 e540 PMID: 36111948
Tomas et al (2019) The Environment Is a Key Factor in Determining the Anti-Amyloid Efficacy of EGCG. Biomolecules 9 PMID: 31835741
Kottakis et al (2016) LKB1 loss links serine metabolism to DNA methylation and tumorigenesis. Nature 539 390 PMID: 27799657
Eom et al (2015) Synergistic effect of curcumin on epigallocatechin gallate-induced anticancer action in PC3 prostate cancer cells. Allergy Asthma Immunol Res 48 461 PMID: 25441423
Do you know of a great paper that uses EGCG from Tocris? Please let us know.
Reviews for EGCG
Average Rating: 4.3 (Based on 3 Reviews.)
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Filter by:
We got expected effect on cells.
By
Anonymous on 04/03/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Primary pancreatic ductal epithelial cells
10 μM EGCG
Showed no visible purine elusions..
By
Scott Scarneo on 02/06/2019
Assay Type: In Vitro
Species: Human
This was used to elute purine binding proteins from cell lysate. The drug gave no visible bands from eluted proteins examined on silver stain.
EGCG.
By
Anonymous on 05/17/2018
Species: Human
Has expected effect on cells.