5-Ethynyl-2'-deoxyuridine

Cat. No. 7207 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Thymidine analog for labeling DNA; can be linked to a fluorophore by click chemistry

化学名： 5-ethynyl-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione

纯度： ≥95% (HPLC)

文献 (2)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for 5-Ethynyl-2'-deoxyuridine

5-Ethynyl-2'-deoxyuridine is a thymidine analog for labeling DNA. Can be linked to an azide fluorophore, such as 3-Azido-7-hydroxycoumarin (Cat. No. 7664) through click chemistry and used to detect DNA synthesis in proliferating cells in vivo and in vitro. Detects very low levels of cell proliferation in tissues with low cellular turnover. 5-Ethynyl-2'-deoxyuridine suppresses cell proliferation in osteosarcoma and glioblastoma cell lines, induces a 'futile' DNA repair cycle and induces DNA strand breaks and apoptosis at a higher rate than Temozolomide (Cat. No. 2706).

技术数据 for 5-Ethynyl-2'-deoxyuridine

分子量	252.23
公式	C₁₁H₁₂N₂O₅
储存	Store at -20°C
纯度	≥95% (HPLC)
CAS Number	61135-33-9
PubChem ID	472172
InChI Key	CDEURGJCGCHYFH-DJLDLDEBSA-N
Smiles	O=C1N([C@@]2(O[C@H](CO)[C@@H](O)C2)[H])C=C(C#C)C(=O)N1

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for 5-Ethynyl-2'-deoxyuridine

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	25.22	100

制备储备液 for 5-Ethynyl-2'-deoxyuridine

以下数据基于产品分子量 252.23。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	3.96 mL	19.82 mL	39.65 mL
5 mM	0.79 mL	3.96 mL	7.93 mL
10 mM	0.4 mL	1.98 mL	3.96 mL
50 mM	0.08 mL	0.4 mL	0.79 mL

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产品说明书 for 5-Ethynyl-2'-deoxyuridine

分析证书/产品说明书

化学品安全技术说明书

参考文献 for 5-Ethynyl-2'-deoxyuridine

参考文献是支持产品生物活性的出版物。

Salic & Mitchison (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc.Natl.Acad.Sci.U.S.A. 105 2415 PMID: 18272492

Wang et al (2022) Nucleotide excision repair removes thymidine analog 5-ethynyl-2'-deoxyuridine from the mammalian genome. Proc.Natl.Acad.Sci.U.S.A. 119 e2210176119 PMID: 35994676

If you know of a relevant reference for 5-Ethynyl-2'-deoxyuridine, please let us know.

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