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Submit Review3-Azido-7-hydroxycoumarin
Description: Click-reactive fluorescent dye. Suitable for live cell imaging
Chemical Name: 3-Azido-7-hydroxy-2H-1-benzopyran-2-one
Purity: ≥95% (HPLC)
Biological Activity for 3-Azido-7-hydroxycoumarin
Key information: 3-Azido-7-hydroxycoumarin is a water-soluble fluorogenic fluorescent dye. Supplied with an azide reactive handle for click chemistry. It emits intense fluorescence only after 'click' reaction when it forms triazoles at the site of alkyne modified biomolecules. Suitable for live-cell imaging.
Application: Fluorescence microscopy. Can be used to label DNA in proliferating cells following 5-Ethynyl-2'-deoxyuridine (Cat.No. 7207) incorporation. Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 404 nm and 477 nm, respectively, after click reaction.
Optical Data for 3-Azido-7-hydroxycoumarin
| Emission Color | Blue |
|---|---|
| λabs | 404 nm |
| λem | 477 nm |
| Application | Fluorescence Microscopy |
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Technical Data for 3-Azido-7-hydroxycoumarin
| M. Wt | 203.15 |
| Formula | C9H5N3O3 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 817638-68-9 |
| PubChem ID | 25127608 |
| InChI Key | KKJNQKVCZCNJDI-UHFFFAOYSA-N |
| Smiles | [N-]=[N+]=NC1=CC=2C=CC(O)=CC2OC1=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for 3-Azido-7-hydroxycoumarin
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 20.32 | 100 | |
| DMF | 20.32 | 100 |
Preparing Stock Solutions for 3-Azido-7-hydroxycoumarin
The following data is based on the product molecular weight 203.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.92 mL | 24.61 mL | 49.22 mL |
| 5 mM | 0.98 mL | 4.92 mL | 9.84 mL |
| 10 mM | 0.49 mL | 2.46 mL | 4.92 mL |
| 50 mM | 0.1 mL | 0.49 mL | 0.98 mL |
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Product Datasheets for 3-Azido-7-hydroxycoumarin
References for 3-Azido-7-hydroxycoumarin
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Keywords: 3-Azido-7-hydroxycoumarin, 3-Azido-7-hydroxycoumarin supplier, 3azido7hydroxycoumarin, fluorescence, triazoles, Fluorescent, fluorogenic, dyes, dye, imaging, Click, Reactive, Click-Reactive, alkyne, Dyes, 7664, Tocris Bioscience
Citations for 3-Azido-7-hydroxycoumarin
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