5-Ph-IAA

Cat. No. 7392 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Selective and potent TAG Degrader for auxin-inducible degron 2 system

化学名： 5-Phenyl-1H-indole-3-acetic acid

纯度： ≥98% (HPLC)

文献 (3)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for 5-Ph-IAA

5-Ph-IAA is a selective and potent "Bump & Hole" TAG Degrader (DC_50,6h = 17.0 nM, T_1/2 = 62.3 min), for use within an auxin-inducible degron (AID2) system. 5-Ph-IAA is an analog (the “Bump”) of natural auxin that induces rapid degradation of a target protein tagged with mAID F-box protein (the "Hole" or mini AID), and recruits a mutant E3 ligase complex, OsTIR1^(F74G). The induced degradation is reversible 3 h after washout with fresh medium. 5-Ph-IAA shows rapid depletion of mAID-tagged nuclear and cytoplasmic proteins in proliferating and non-proliferating mammalian cells with no detectable basal degradation, lower cytotoxicity level, increased potency and faster degradation of the reporter in comparison with the conventional AID system. The AID2 system also works in budding yeast and fission yeast, and it can control protein expression in multiple mouse organs in vivo.

5-Ph-IAA-AM (Cat. No. 7893), an analog of 5-Ph-IAA, is also available.

Plasmid vectors for the AAV transfection and CRISPR-mediated knock-in of OsTIR1^F74Gand mAID tag are available from Addgene.

技术数据 for 5-Ph-IAA

分子量	251.29
公式	C₁₆H₁₃NO₂
储存	Store at -20°C
纯度	≥98% (HPLC)
CAS Number	168649-23-8
PubChem ID	15207491
InChI Key	ACCCWYHBDGKDKN-UHFFFAOYSA-N
Smiles	OC(CC1=CNC2=C1C=C(C3=CC=CC=C3)C=C2)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for 5-Ph-IAA

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
	DMSO	25.13	100
	ethanol	25.13	100

制备储备液 for 5-Ph-IAA

以下数据基于产品分子量 251.29。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	3.98 mL	19.9 mL	39.79 mL
5 mM	0.8 mL	3.98 mL	7.96 mL
10 mM	0.4 mL	1.99 mL	3.98 mL
50 mM	0.08 mL	0.4 mL	0.8 mL

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产品说明书 for 5-Ph-IAA

分析证书/产品说明书

化学品安全技术说明书

参考文献 for 5-Ph-IAA

参考文献是支持产品生物活性的出版物。

Yeung et al (2021) One-step multiplex toolkit for efficient generation of conditional gene silencing human cell lines. Mol.Biol.Cell 32 1320 PMID: 33979199

Yesbolatova et al (2020) The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat.Commun. 11 5701 PMID: 33177522

Zhang et al (2021) An improved auxin-inducible degron system for fission yeast. G3 Genes|Genomes|Genetics 12 PMID: 34849776

If you know of a relevant reference for 5-Ph-IAA, please let us know.

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关键词: 5-Ph-IAA, 5-Ph-IAA supplier, Bump, Hole, auxin-inducible, degron, AID2, AID, TAG, Degrades, Degraders, degradation, target, fusion, protein, mAID, Degradation, Platform, Molecular, Glues, 7392, Tocris Bioscience

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该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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TPD and Induced Proximity Research Product GuideUpdated

This brochure highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation and Induced Proximity research, including:

Active Degraders
TAG Degradation Platform
Degrader Building Blocks
Assays for Protein Degradation
Induced Proximity Tools

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia

Validating Targets for TPD Using dTAG Poster

The dTAG platform offers a generalizable strategy to degrade, in principle, any intracellular protein of interest (POI) and is a useful strategy for exploration and validation of targets. This poster presents a workflow solution for target validation, from custom TAG knock-in cell-lines for your POI, different dTAG Degraders to knockdown you POI, to automated assays for protein degradation using Simple Western^TM instruments. Data are also presented on the use of an antibody that recognizes the TAG domain (FKBP12^F36V) for detection of degradation. Presented at TPD Europe, March 2022, London, UK.