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ALDH Detection Reagent - BAAA-DA

Cat. No. 7556 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Fluorescent ALDH substrate and detection reagent. Used for: identification of cells with high ALDH activity. Application: flow cytometry, fluorescent-activated cell sorting (FACS)
化学名： (T-4)-[N-(2,2-Diethoxyethyl)-5-[(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrole-2-propanamidato-κN1]difluoroboron
纯度： ≥95% (HPLC)
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生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for ALDH Detection Reagent - BAAA-DA

Key information: ALDH Detection Reagent - BAAA-DA is a fluorescent substrate of aldehyde dehydrogenase (ALDH).

Used for: can be used in flow cytometry to sort cancer stem cells with high ALDH levels, and to isolate human hematopoietic progenitor cells. Can be used with efflux inhibitors such as Verapamil (Cat. No. 0654), Probenecid (Cat. No. 4107) or 2-deoxy-D-glucose (Cat. No. 4515) to block multi-drug resistance efflux.

Application: flow cytometry, fluorescent-activated cell sorting (FACS).

Properties and Photophysical Data: BAAA-DA is converted under acidic conditions to BAAA. BAAA, cell-permeant, is converted by intracellular ALDH into carboxylate ion amino acetate (BAA) and used to identify cells with high ALDH activity. Excitation and emission maxima (λ) are 505 nm and 512 nm, respectively; extinction coefficient = 74,595 M-1cm-1.

Optical Data for ALDH Detection Reagent - BAAA-DA

ALDH Detection Reagent - BAAA-DA Dye Spectra
λabs 505 nm
λem 512 nm
Extinction Coefficient (ε) 74595 M-1cm-1

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技术数据 for ALDH Detection Reagent - BAAA-DA

分子量 407.27
公式 C20H28BF2N3O3
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 247069-93-8
PubChem ID 91759536
InChI Key LHPURSCMUYRRRM-UHFFFAOYSA-N
Smiles CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(NCC(OCC)OCC)=O)C=CC3=C2

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for ALDH Detection Reagent - BAAA-DA

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 4.07 10

制备储备液 for ALDH Detection Reagent - BAAA-DA

以下数据基于产品分子量 407.27。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 24.55 mL 122.77 mL 245.54 mL
0.5 mM 4.91 mL 24.55 mL 49.11 mL
1 mM 2.46 mL 12.28 mL 24.55 mL
5 mM 0.49 mL 2.46 mL 4.91 mL

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产品说明书 for ALDH Detection Reagent - BAAA-DA

分析证书/产品说明书
化学品安全技术说明书

参考文献 for ALDH Detection Reagent - BAAA-DA

参考文献是支持产品生物活性的出版物。

Storms et al (1999) Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity. Proc.Natl.Acad.Sci.U.S.A. 96 9118 PMID: 10430905

If you know of a relevant reference for ALDH Detection Reagent - BAAA-DA, please let us know.

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关键词: ALDH Detection Reagent - BAAA-DA, ALDH Detection Reagent - BAAA-DA supplier, Aldefluor, ALDH, detection, reagent, aldehyde, dehydrogenase, substrate, detector, aminoacetaldehyde, BDY, Bodipy, flow, cytometry, BAAA-DA, Enzyme, Probes, and, Substrates, 7556, Tocris Bioscience

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