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Submit Review2-Deoxy-D-glucose
Description: Non-metabolizable glucose analog; inhibits glucose metabolism
Chemical Name: 2-Deoxy-D-arabino-hexose
Biological Activity for 2-Deoxy-D-glucose
2-Deoxy-D-glucose is a non-metabolizable glucose analog. Inhibits phosphorylation of glucose by hexokinase; causes depletion of cellular ATP. Also inhibits phosphoglucose isomerase (PGI) competitively. Causes cell cycle inhibition and cell death in in vitro models of hypoxia; blocks tumor cell growth in animal models. Also shown to induce the unfolded protein response (UPR). 2-Deoxy-D-glucose disrupts N-glycan expression on the surface of pancreatic adenocarcinoma cells. 2-Deoxy-D-glucose enhances chimeric antigen receptor T cell activity in different xenograft mouse models of pancreatic adenocarcinoma cells.
Technical Data for 2-Deoxy-D-glucose
| M. Wt | 164.16 |
| Formula | C6H12O5 |
| Storage | Store at +4°C |
| CAS Number | 154-17-6 |
| PubChem ID | 439268 |
| InChI Key | PMMURAAUARKVCB-CERMHHMHSA-N |
| Smiles | OC1C[C@@H](O)[C@H](O)[C@@H](CO)O1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for 2-Deoxy-D-glucose
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 16.42 | 100 | |
| DMSO | 16.42 | 100 |
Preparing Stock Solutions for 2-Deoxy-D-glucose
The following data is based on the product molecular weight 164.16. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 6.09 mL | 30.46 mL | 60.92 mL |
| 5 mM | 1.22 mL | 6.09 mL | 12.18 mL |
| 10 mM | 0.61 mL | 3.05 mL | 6.09 mL |
| 50 mM | 0.12 mL | 0.61 mL | 1.22 mL |
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Product Datasheets for 2-Deoxy-D-glucose
References for 2-Deoxy-D-glucose
References are publications that support the biological activity of the product.
Kang and Hwang (2006) 2-deoxyglucose: an anticancer and antiviral therapeutic, but not any more a low glucose mimetic. Life Sci. 78 1392 PMID: 16111712
Maher et al (2004) Greater cell cycle inhibition and cytotoxicity induced by 2-deoxy-D-glucose in tumor cells treated under hypoxic vs aerobic conditions. Cancer Chemother.Pharmacol. 53 116 PMID: 14605866
Ralser et al (2008) A catabolic block does not sufficiently explain how 2-deoxy-D-glucose inhibits cell growth. Proc.Natl.Acad.Sci.USA 105 17807 PMID: 19004802
Greco et al (2022) Disrupting N-glycan expression on tumor cells boosts chimeric antigen receptor T cell efficacy against solid malignancies. Sci.Transl.Med. 14 3072 PMID: 35044789
If you know of a relevant reference for 2-Deoxy-D-glucose, please let us know.
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Keywords: 2-Deoxy-D-glucose, 2-Deoxy-D-glucose supplier, non-metabolizable, hexokinase, substrates, hypoxia, hypoxic, cell, growth, inhibitors, inhibits, inhibition, Hexokinases, Enzyme, Substrates, /, Activators, Other, ER, stress/UPR, 4515, Tocris Bioscience
2 Citations for 2-Deoxy-D-glucose
Citations are publications that use Tocris products. Selected citations for 2-Deoxy-D-glucose include:
Lin et al (2017) Lactate-activated macrophages induced aerobic glycolysis and epithelial-mesenchymal transition in breast cancer by regulation of CCL5-CCR5 axis: a positive metabolic feedback loop. Oncotarget 8 110426 PMID: 29299159
Beas et al (2020) A ventrolateral medulla-midline thalamic circuit for hypoglycemic feeding. Nat.Commun. 11 6218 PMID: 33277492
Do you know of a great paper that uses 2-Deoxy-D-glucose from Tocris? Please let us know.
Reviews for 2-Deoxy-D-glucose
Average Rating: 4.7 (Based on 3 Reviews.)
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Good quality for in vitro study.
By
Anonymous on 03/07/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: MDA-MB-231
2-DG (10mM) was used to inhibit the glycolytic pathway in MDA-MB-231 cell lines.
Blockade of glycolysis at 5 mM enhances lipid peroxidation.
By
Anonymous on 02/27/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: human cancer lines
Used to inhibit glycolysis and influence activity of a lipid peroxidation enzyme. Good activity was observed at 5 mM with the majority of cell lines surviving. We found a few cancer cell lines where 5 uM was cytotoxic
Dissolved in PBS to 500 mM. Suggest ordering a large size as it goes by quickly at 5mM.
Glycolysis block.
By
Anonymous on 01/31/2018
Assay Type: Ex Vivo
Species: Mouse
Cell Line/Tissue: acute brain slice
Used in brain slices to block glycolysis. Effect observed on cells within minutes of bulk application, as expected.
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