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BRD 0539

Cat. No. 7153 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Cell permeable and reversible Cas9 inhibitor
化学名： (3aR,4R,9bR)-8-(2-Fluorophenyl)-2,3,3a,4,5,9b-hexahydro-1-[(4-methylphenyl)sulfonyl]-1H-pyrrolo[3,2-c]quinoline-4-methanol
纯度： ≥98% (HPLC)
说明书
引用文献
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文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for BRD 0539

BRD 0539 is a cell-permeable and reversible SpCas9 (Streptococcus pyogenes Cas9) inhibitor. BRD 0539 shows dose-dependent SpCas9 inhibition in an in vitro DNA cleavage assay (apparent IC50 = 22 μM) and in an eGFP disruption assay (apparent EC50 = 11 μM). Allows dose and temporal control of SpCas9-based systems.

许可信息

Sold under licence from the Broad Institute

技术数据 for BRD 0539

分子量 452.54
公式 C25H25FN2O3S
储存 Store at -20°C
纯度 ≥98% (HPLC)
CAS Number 1403838-79-8
PubChem ID 54666326
InChI Key CZOIXFMISSBIJL-DCEDVJGZSA-N
Smiles [H][C@]12CCN([C@@]1([H])C1=CC(=CC=C1N[C@H]2CO)C1=C(F)C=CC=C1)[S](=O)(=O)C1=CC=C(C)C=C1

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for BRD 0539

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 45.25 100
ethanol 45.25 100

制备储备液 for BRD 0539

以下数据基于产品分子量 452.54。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 2.21 mL 11.05 mL 22.1 mL
5 mM 0.44 mL 2.21 mL 4.42 mL
10 mM 0.22 mL 1.1 mL 2.21 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

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参考文献 for BRD 0539

参考文献是支持产品生物活性的出版物。

Maji et al (2019) A high-throughput platform to identify small-molecule inhibitors of CRISPR-Cas9. Cell 177 1067 PMID: 31051099

If you know of a relevant reference for BRD 0539, please let us know.

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关键词: BRD 0539, BRD 0539 supplier, BRD0539, inhibits, inhibitors, Cas9, SpCas9, reversible, specific, CRISPR, hematopoietic, haematopoietic, stem, cells, HSC, Reagents, Hematopoietic, Stem, Cells, 7153, Tocris Bioscience

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