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Digoxigenin NHS ester

Cat. No. 7784 Submit a Review Datasheet / COA / SDS
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Description: NHS ester hapten for labeling amino groups
Chemical Name: (3β,5β,12β)-3-[2-[[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]amino]-2-oxoethoxy]-12,14-dihydroxycard-20(22)-enolide
Purity: ≥80% (HPLC)
Datasheet
Citations
Reviews
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Digoxigenin NHS ester

Digoxigenin NHS ester is an NHS ester hapten for labeling amino groups within biomolecules (oligonucleotides or proteins). Digoxigenin NHS ester is also used for in situ hybridization.

Technical Data for Digoxigenin NHS ester

M. Wt 658.79
Formula C35H50N2O10
Storage Store at -20°C
Purity ≥80% (HPLC)
CAS Number 129273-26-3
InChI Key KHNDABJZSPPYLE-FUGFVFQCSA-N
Smiles O[C@@]12[C@@]([C@@H](C(CO3)=CC3=O)CC1)([C@@H](C[C@@]4([H])[C@@]2([H])CC[C@@]5([H])[C@@]4(CC[C@@H](C5)OCC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)C)O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Digoxigenin NHS ester

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 65.88 100
DMF 65.88 100

Preparing Stock Solutions for Digoxigenin NHS ester

The following data is based on the product molecular weight 658.79. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.52 mL 7.59 mL 15.18 mL
5 mM 0.3 mL 1.52 mL 3.04 mL
10 mM 0.15 mL 0.76 mL 1.52 mL
50 mM 0.03 mL 0.15 mL 0.3 mL

Molarity Calculator

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Product Datasheets for Digoxigenin NHS ester

Certificate of Analysis / Product Datasheet
Safety Datasheet

References for Digoxigenin NHS ester

References are publications that support the biological activity of the product.

Heyduk et al (2018) Peptide ligand-based ELISA reagents for antibody detection. Anal.Biochem. 559 55 PMID: 30130491

If you know of a relevant reference for Digoxigenin NHS ester, please let us know.

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Citations for Digoxigenin NHS ester

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