主页 / Product Type / 荧光成像 / Fluorescent Probes for Imaging Bacteria / EDA-DA

EDA-DA

Cat. No. 7714 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Unnatural dipeptide building block with alkyne group for functionalizing peptidoglycan
化学名： N-(4,4,5,5-Tetradehydro-D-norvalyl)-D-alanine trifluoroacetate
纯度： ≥95% (HPLC)
说明书
引用文献
评论
文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for EDA-DA

EDA-DA is an unnatural dipeptide building block (an ethynyl-D-alanine and a D-alanine). It incorporates a biorthogonal alkyne group into peptidoglycan (PG) through MurF in the cytoplasmic pathway, which enables selective labeling via a click-chemistry reaction. EDA-DA allows labeling of PG in Gram-positive (B. subtilis), Gram-negative (E. coli and C. trachomatis), Mycobacterium (M. smegmatis) and moss plastids (P. patens) with azide modified fluorescent dyes such as Alexa Fluor 488.

技术数据 for EDA-DA

分子量 298.22
公式 C8H12N2O3.CF3CO2H
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 87156-01-2
InChI Key BRIXKPONTJDKNO-KGZKBUQUSA-N
Smiles C[C@H](C(O)=O)NC([C@@H](CC#C)N)=O.FC(F)(F)C(O)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for EDA-DA

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 29.82 100
water 29.82 100

制备储备液 for EDA-DA

以下数据基于产品分子量 298.22。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 3.35 mL 16.77 mL 33.53 mL
5 mM 0.67 mL 3.35 mL 6.71 mL
10 mM 0.34 mL 1.68 mL 3.35 mL
50 mM 0.07 mL 0.34 mL 0.67 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

参考文献 for EDA-DA

参考文献是支持产品生物活性的出版物。

Cheung et al (1983) Chloralanyl and propargylglycyl dipeptides. Suicide substrate containing antibacterials. J.Med.Chem. 26 1733 PMID: 6417332

Liechti et al (2013) A new metabolic cell-wall labelling method reveals peptidoglycan in Chlamydia trachomatis. Nature 506 507 PMID: 24336210

Hirano et al (2016) Moss chloroplasts are surrounded by a peptidoglycan wall containing D-amino acids. Plant Cell 28 1521 PMID: 27325639

Kuru et al (2019) Mechanisms of incorporation for D-amino acid probes that target peptidoglycan biosynthesis. ACS Chem.Biol. 14 2745 PMID: 31743648

Banahene et al (2022) Chemical reporters for bacterial glycans: development and applications. Chem.Rev. 122 3336 PMID: 34905344

If you know of a relevant reference for EDA-DA, please let us know.

按标靶查看相关产品

关键词: EDA-DA, EDA-DA supplier, unnatural, dipeptide, ethynyl-D-alanine, alkyne, selective, labeling, peptidoglycan, Gram-positive, Gram-negative, Mycobacterium, bacteria, Fluorescent, Probes, for, Imaging, Bacteria, Unnatural, Amino, Acids, 7714, Tocris Bioscience

篇 EDA-DA 的引用文献

引用文献是使用了 Tocris 产品的出版物。

目前没有 EDA-DA 的引用文献。 您是否知道使用了 Tocris EDA-DA 的优秀论文？ 请告知我们.

EDA-DA 的评论

目前没有该产品的评论。 Be the first to review EDA-DA and earn rewards!

Have you used EDA-DA?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals