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Submit ReviewGemcitabine hydrochloride
说明： DNA synthesis inhibitor
化学名： (+)-2'-Deoxy-2',2'-difluorocytidine hydrochloride
纯度： ≥99% (HPLC)
生物活性 for Gemcitabine hydrochloride
Gemcitabine hydrochloride is a deoxycytidine analog that inhibits DNA synthesis. Metabolized to form gemcitabine triphosphate (dFdCTP) and gemcitabine diphosphate (dFdCDP). dFdCTD inhibits ribonucleotide reductase causing a reduction in cellular nucleotides. dFdCTP is incorporated in DNA resulting in DNA strand termination. Displays antitumor activity in vitro and in vivo. Inhibits replication of MERS-CoV and SARS-CoV in vitro (EC50 values are 1.216 and 4.957 μM, respectively).
化合物库 for Gemcitabine hydrochloride
Gemcitabine hydrochloride is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Antiviral Library and Tocriscreen FDA-Approved Drugs. 了解 Tocris 化合物库的更多信息。
技术数据 for Gemcitabine hydrochloride
| 分子量 | 299.66 |
| 公式 | C9H11F2N3O4.HCl |
| 储存 | Desiccate at +4°C |
| 纯度 | ≥99% (HPLC) |
| CAS Number | 122111-03-9 |
| PubChem ID | 60749 |
| InChI Key | OKKDEIYWILRZIA-OSZBKLCCSA-N |
| Smiles | OC[C@@H]1[C@@H](O)C(F)(F)[C@H](N2C(N=C(N)C=C2)=O)O1.Cl |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Gemcitabine hydrochloride
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| water | 29.97 | 100 | |
| DMSO | 5.99 | 20 |
制备储备液 for Gemcitabine hydrochloride
以下数据基于产品分子量 299.66。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.34 mL | 16.69 mL | 33.37 mL |
| 5 mM | 0.67 mL | 3.34 mL | 6.67 mL |
| 10 mM | 0.33 mL | 1.67 mL | 3.34 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.67 mL |
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产品说明书 for Gemcitabine hydrochloride
参考文献 for Gemcitabine hydrochloride
参考文献是支持产品生物活性的出版物。
Hertel et al (1990) Evaluation of the antitumor activity of gemcit. (2',2'-Difluoro-2'-deoxycytidine). Cancer Res. 50 4417 PMID: 2364394
Heinemann et al (1995) Gemcitabine: a modulator of intracellular nucleotide and deoxynucleotide metabolism. Semin.Oncol. 22 11 PMID: 7481839
Plunkett et al (1995) Preclinical characteristics of gemcit. Anticancer Drugs 6 7 PMID: 8718419
Dyall et al (2014) Repurposing of clinically developed drugs for treatment of Middle East Respiratory Syndrome coronavirus infection. Antimicrob.Agents.Chemother. 58 4885 PMID: 24841273
If you know of a relevant reference for Gemcitabine hydrochloride, please let us know.
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关键词: Gemcitabine hydrochloride, Gemcitabine hydrochloride supplier, DNA, synthesis, inhibitors, inhibits, Ribonucleotide, reductases, chemotherapeutics, coronaviruses, COVID-19, SARS-CoV, MERS, severe, acute, respiratory, syndrome, DNA,, RNA, and, Protein, Synthesis, Reductase, Coronavirus, 3259, Tocris Bioscience
14 篇 Gemcitabine hydrochloride 的引用文献
引用文献是使用了 Tocris 产品的出版物。 Gemcitabine hydrochloride 的部分引用包括：
Marchetti et al (2018) Targeting Multiple Effector Pathways in Pancreatic Ductal Adenocarcinoma with a G-Quadruplex-Binding Small Molecule. J Med Chem 61 2500 PMID: 29356532
Viotti et al (2018) SUV420H2 is an epigenetic regulator of epithelial/mesenchymal states in pancreatic cancer. J Cell Biol 217 763 PMID: 29229751
Okita et al (2015) Contrasting Effects of the Cytotoxic Anticancer Drug gemcit. and the EGFR Tyrosine Kinase Inhibitor gefi. on NK Cell-Mediated Cytotoxicity via Regulation of NKG2D Ligand in Non-Small-Cell Lung Cancer Cells. PLoS One 10 e0139809 PMID: 26439264
Dolman et al (2015) DNA-Dependent Protein Kinase As Molecular Target for Radiosensitization of Neuroblastoma Cells. PLoS One 10 e0145744 PMID: 26716839
Jones et al (2014) BRCA2 and RAD51 promote double-strand break formation and cell death in response to gemcit. PLoS One 13 2412 PMID: 25053826
Truman et al (2015) Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveal chaperone-dependent regulation of ribonucleotide reductase. J Proteomics 112 285 PMID: 25452130
Song et al (2013) Plk1 phosphorylation of orc2 and hbo1 contributes to gemcit. resistance in pancreatic cancer. Mol Cancer Ther 12 58 PMID: 23188630
Clouser et al (2013) Characterization of permeability, stability and anti-HIV-1 activity of decit. and gemcit. divalerate prodrugs. Antivir Chem Chemother 23 223 PMID: 23994876
Chan et al (2013) A simple high-content cell cycle assay reveals frequent discrepancies between cell number and ATP and MTS proliferation assays. PLoS One 8 e63583 PMID: 23691072
Li et al (2016) Plk1 inhibition enhances the efficacy of gemcit. in human pancreatic cancer. Cell Cycle 15 711 PMID: 26890815
Veroli et al (2015) An automated fitting procedure and software for dose-response curves with multiphasic features. Mol Cancer Ther 5 14701 PMID: 26424192
Bapiro et al (2014) Gemcitabine diphosphate choline is a major metabolite linked to the Kennedy pathway in pancreatic cancer models in vivo. Br J Cancer 111 318 PMID: 24874484
Slee et al (2014) Selective inhibition of pancreatic ductal adenocarcinoma cell growth by the mitotic MPS1 kinase inhibitor NMS-P715. Mol Cancer Ther 13 307 PMID: 24282275
Mayor-López et al (2014) Comparative Study of 17-AAG and NVP-AUY922 in Pancreatic and Colorectal Cancer Cells: Are There Common Determinants of Sensitivity? Transl Oncol 7 590 PMID: 25389454
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Gemcitabine hydrochloride 的评论
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Great product.
By
Anonymous on 07/28/2019
分析类型： In Vitro
种属： Human
细胞系/组织： Jurkat Cells
The compound was used as a control in a study where we determined the effect of our compounds on ribonucleotide reductase enzyme.
The concentration used was 20uM.
Gemcitabine effectively kills growth of melanoma cell line in vitro.
By
Anonymous on 02/14/2019
分析类型： In Vitro
种属： Human
Gemcitabine was used at a 20 uM concentration for in vitro culture with SK-MEL-28 and cell viability was monitored over time with live imaging. Arrow indicates when compound was added to media.
该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Cell Cycle and DNA Damage Research Product Guide
This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:
- Cell Cycle and Mitosis
- DNA Damage Repair
- Targeted Protein Degradation
- Ubiquitin Proteasome Pathway
- Chemotherapy Targets
Huntington's Disease Research Product Guide
This product guide provides a background to Huntington's disease research and lists around 100 products for the study of:
- Somatic Instability
- Proteolysis and Inclusion Bodies
- Transcriptional Dysregulation
- Mitochondrial Dysfunction
- Nuclear-Cytoplasmic Transport Interference
- Excitotoxicity
- Stem Cells