主页 / Product Type / 荧光成像 / Fluorescent Probes for Imaging Bacteria / HADA

HADA

Cat. No. 6647 4 篇引用文献 1 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Blue fluorescent D-amino acid for labeling peptidoglycans in live bacteria
化学名： 3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl)carbonyl]amino]-D-alanine hydrocholoride
纯度： ≥98% (HPLC)
说明书
引用文献 (4)
评论 (1)
文献 (1)
生物活性 Scientific Data 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for HADA

HADA is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Results in strong peripheral and septal labeling of diverse bacterial cell populations without affecting growth rates. Displays mitochondrial outer membrane permeability. Excitation/emission λ ~405/450 nm.

Scientific Data

Investigating mycobacteria cell wall formation using FDAA HADA. View Larger

Investigating mycobacteria cell wall formation using FDAA HADA. Investigating mycobacteria cell wall formation using fluorescent D-amino acid HADA. Image showing FDAA HADA incorporation in log-phase growth of WT, delta L,D-transpeptidases (LDT) and delta L,D-transpeptidases (LDT) comp cells. Scale = 5 μm. Baranowski et al show FDAAs are incorporated asymmetrically by L,D-transpeptidases. For more information on this work and to request full protocols read Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. Elife 16 e37516 PMID: 30324906

Using FDAAs HADA and RADA (Cat. No 6649) to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time. View Larger

Using FDAAs HADA and RADA to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time. Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA (Catalog # 6649) chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale = 5 μm. For more information on this work and to request full protocols please read García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 10 e37243 PMID: 30198841

Optical Data for HADA

HADA Dye Spectra
Emission Color Blue
λabs 405 nm
λem 450 nm
Extinction Coefficient (ε) 36,700 M-1cm-1
Application Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for HADA

分子量 328.71
公式 C13H12N2O6.HCl
储存 Store at -20°C
纯度 ≥98% (HPLC)
CAS Number 2253733-10-5
PubChem ID 137919867
InChI Key PLVOEKQNBIXHDI-SBSPUUFOSA-N
Smiles N[C@@H](C(O)=O)CNC(C1=CC(C=CC(O)=C2)=C2OC1=O)=O.Cl

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for HADA

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 32.87 100

制备储备液 for HADA

以下数据基于产品分子量 328.71。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 3.04 mL 15.21 mL 30.42 mL
5 mM 0.61 mL 3.04 mL 6.08 mL
10 mM 0.3 mL 1.52 mL 3.04 mL
50 mM 0.06 mL 0.3 mL 0.61 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

产品说明书 for HADA

分析证书/产品说明书
选择另一批次：
化学品安全技术说明书

参考文献 for HADA

参考文献是支持产品生物活性的出版物。

Kuru et al (2012) In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew.Chem.Int.Ed. 51 12519 PMID: 23055266

Hsu et al (2017) Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 8 6313 PMID: 28989665

Botella et al (2017) Distinct spatiotemporal dynamics of peptidoglycan synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis. Mbio. 8 e01183 PMID: 28900018

If you know of a relevant reference for HADA, please let us know.

按标靶查看相关产品

关键词: HADA, HADA supplier, fluorescent, D-amino, acid, FDAA, peptidoglycans, labeling, bacteria, labels, labelling, blue, Unnatural, Amino, Acids, Fluorescent, Probes, for, Imaging, Bacteria, 6647, Tocris Bioscience

4 篇 HADA 的引用文献

引用文献是使用了 Tocris 产品的出版物。 HADA 的部分引用包括：

Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. ELife 7 PMID: 30324906

Garcia-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria ELife 7 PMID: 30198841

Mariana et al (2022) The Staphylococcus aureus cell division protein, DivIC, interacts with the cell wall and controls its biosynthesis. Commun Biol 5 1228 PMID: 36369270

Marc et al (2021) Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth. Elife 10 PMID: 34605403

您是否知道使用了 Tocris HADA 的优秀论文？ 请告知我们.

HADA 的评论

平均评分: 5 (Based on 1 Review.)

5 星
100%
4 星
0%
3 星
0%
2 星
0%
1 星
0%

Have you used HADA?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Great for labelling bacterial cell walls..
By Patrick Moynihan on 05/11/2020
分析类型： In Vitro
种属： Other

We use this product to label the peptidoglycan of growing mycobacterial species. We generally follow published protocols for this, of which there are several. This will label regions of active peptidoglycan synthesis in the cell.

review image

该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals