HEPES

Cat. No. 3173 1 次引用 3 条评论提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Multipurpose HEPES buffer; used in cell culture

化学名： 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

纯度： ≥99.5%

说明书

引用文献 (1)

实验方案 (1)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for HEPES

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

For more information about how HEPES may be used, see our protocol:

3D Culture of Lung Alveolar Cells

技术数据 for HEPES

分子量	238.3
公式	C₈H₁₈N₂O₄S
储存	Store at RT
纯度	≥99.5%
CAS Number	7365-45-9
PubChem ID	23830
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Smiles	OCCN1CCN(CCS(=O)(O)=O)CC1

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for HEPES

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	water	238.3	1000

制备储备液 for HEPES

以下数据基于产品分子量 238.3。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
10 mM	0.42 mL	2.1 mL	4.2 mL
50 mM	0.08 mL	0.42 mL	0.84 mL
100 mM	0.04 mL	0.21 mL	0.42 mL
500 mM	0.01 mL	0.04 mL	0.08 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

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产品说明书 for HEPES

分析证书/产品说明书

化学品安全技术说明书

参考文献 for HEPES

参考文献是支持产品生物活性的出版物。

Good et al (1966) Hydrogen ion buffers for biological research. Biochemistry 5 467 PMID: 5942950

Medzon and Gedies (1971) Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol. 17 651

If you know of a relevant reference for HEPES, please let us know.

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关键词: HEPES, HEPES supplier, Multipurpose, buffer, Reagents, cell, culture, ph, Buffers,, Solvents, and, Solutions, Organoids, 3173, Tocris Bioscience

1 篇 HEPES 的引用文献

引用文献是使用了 Tocris 产品的出版物。 HEPES 的部分引用包括：

Lerner et al (2010) Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists. J Neurosci 30 2160 PMID: 20147543

您是否知道使用了 Tocris HEPES 的优秀论文？请告知我们.

HEPES 的评论

平均评分: 4.3 (基于 3 条评论。)

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HEPES (10 mM) maintains pH in the experimental buffer for Ca2+ imaging..

By Anonymous on 07/02/2019

分析类型： In Vitro

种属： Human

细胞系/组织： HEK293

HEPES (10 mM) is used to maintain pH 7.4 in the experimental buffer for Ca2+ imaging, consisting of 137 mM NaCl, 5.4 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 10 mM glucose.

Good buffer.

By Anonymous on 08/05/2018

分析类型： Ex Vivo

种属： Mouse

Used to buffer Krebs buffer for pressure myography. Good performance, maintained pH without any problem. I would use it again.

HEPES to buffer pH.

By Anonymous on 01/31/2018