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Submit ReviewHM Janelia Fluor® 526, NHS ester
说明： Fluorogenic spontaneously blinking yellow-emitting dye (Reactive group: NHS ester). Application: dSTORM, SMLSM, STED, SIM and confocal microscopy
别名： HM-JF526 NHS
化学名： 2,5-Dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate
纯度： ≥90% (HPLC)
生物活性 for HM Janelia Fluor® 526, NHS ester
Key information: HM Janelia Fluor® 526, NHS ester is a fluorogenic spontaneously blinking yellow-emitting dye; supplied with an NHS ester reactive group for the labeling of primary amines; hydroxymethyl derivative of JF526. Suitable for live cell imaging.
Application: Suitable for confocal microscopy, super-resolution microscopy (SRM) including dSTORM, STED, SIM and single-molecule localization spectroscopy (SMLSM). HM Janelia Fluor® 526, NHS ester is cell permeable.
Properties and Photophysical Data: The spontaneous blinking properties of HM Janelia Fluor® 526, NHS ester avoids the requirement for strongly reducing dSTORM buffers. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 526 nm and 550 nm, respectively.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was HM Janelia Fluor® 526, SE.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for HM Janelia Fluor® 526, NHS ester
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 526 nm |
| λem | 550 nm |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | dSTORM, SMLSM, STED, SIM, Confocal Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for HM Janelia Fluor® 526, NHS ester
| 分子量 | 645.51 |
| 公式 | C31H21F6N3O6 |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 2376841-30-2 |
| InChI Key | HJUSNAUMCROPEK-UHFFFAOYSA-N |
| Smiles | FC1=C(N2CC(F)(C2)F)C=C3OC4=CC(N5CC(F)(C5)F)=C(C=C4C6(C3=C1)OCC7=CC=C(C(ON8C(CCC8=O)=O)=O)C=C67)F |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for HM Janelia Fluor® 526, NHS ester
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 12.91 | 20 |
制备储备液 for HM Janelia Fluor® 526, NHS ester
以下数据基于产品分子量 645.51。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 7.75 mL | 38.73 mL | 77.46 mL |
| 1 mM | 1.55 mL | 7.75 mL | 15.49 mL |
| 2 mM | 0.77 mL | 3.87 mL | 7.75 mL |
| 10 mM | 0.15 mL | 0.77 mL | 1.55 mL |
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产品说明书 for HM Janelia Fluor® 526, NHS ester
参考文献 for HM Janelia Fluor® 526, NHS ester
参考文献是支持产品生物活性的出版物。
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for HM Janelia Fluor® 526, NHS ester, please let us know.
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关键词: HM Janelia Fluor 526, NHS ester, HM Janelia Fluor 526, NHS ester supplier, Fluorogenic, Spontaneously, blinking, green, emitting, dyes, for, SMLSM, single-molecule, localisation, localization, spectroscopy, srm, super, resolution, microscopy, STORM, dSTORM, STED, HM, Janelia, Fluor, 526-NHS, ester, JF526-NHS, HM-JF526, NHS, Amine, Reactive, Dyes, 7312, Tocris Bioscience
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