Hoechst Janelia Fluor^® 646

Cat. No. 6804 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Fluorogenic, red-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, super resolution microscopy (SRM) (PALM, PAINT), lattice light sheet microscopy

别名： Hoechst JF 646,JF646-Hoechst

化学名： 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(4-(4-(5-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate

纯度： ≥85% (HPLC)

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生物活性 Scientific Data 技术数据溶解性计算器说明书参考文献

生物活性 for Hoechst Janelia Fluor^® 646

Key information: Hoechst Janelia Fluor^® 646 is a fluorogenic red-emitting DNA probe. Suitable for fixed and live-cell imaging.

Application:confocal microscopy, super resolution microscopy (SRM) (including PALM and PAINT), lattice light sheet microscopy.

Properties and Photophysical Data: Hoechst Janelia Fluor^® 646 preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor^® 526 (Cat. No. 7313) for multiplexing experiments. Hoechst Janelia Fluor^® 646 can also be combined with Hoechst Janelia Fluor^® 526 to perform dual-color STED using the same depletion laser (λ_dep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor^® 646 in E. coli cells (~100 nM - 1 μM). Hoechst Janelia Fluor^® 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Excitation and emission maxima (λ) are 655 nm and 670 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M^-1cm^-1.

Hoechst Janelia Fluor^® 646 is fluorogenic and fluoresces only once bound to DNA, enabling hassle-free no-wash experiments.

To measure the absorbance spectrum of Hoechst Janelia Fluo^® 646 we recommend the following solvent: TFE plus 0.1% TFA.

Scientific Data

Confocal Microscopy

Application of Hoechst Janelia Fluor<sup>®</sup> 646 in U2OS cells.

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Application of Hoechst Janelia Fluor^® 646 in U2OS cells. Fixed U2OS cells were stained with Hoechst Janelia Fluor^® 646 (1 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 633 nm / emission: 638–759 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Hoechst Janelia Fluor^® 646

Hoechst Janelia Fluor<sup>®</sup> 646 Dye Spectra

Emission Color	Red
Brightness
λ_abs	655 nm
λ_em	670 nm
Extinction Coefficient (ε)	152,000 M^-1cm^-1
Quantum Yield (φ)	0.54
Closest Laser line	640 nm
Cell Permeable	Yes
Application	Super Resolution Microscopy (PALM, PAINT), Confocal Microscopy, Lattice Light Sheet Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Hoechst Janelia Fluor^® 646

分子量	1119.39
公式	C₆₄H₇₀N₁₀O₇Si
储存	Store at -20°C
纯度	≥85% (HPLC)
CAS Number	2462011-60-3
InChI Key	DTAPBNBSCGEQMS-UHFFFAOYSA-N
Smiles	C[Si]1(C)C2=C/C(C=CC2=C(C3=CC(C(NCCOCCOCCNC(CCCOC4=CC=C(C5=NC6=C(C=C(C=C6)C7=NC8=C(C=CC(N9CCN(C)CC9)=C8)N7)N5)C=C4)=O)=O)=CC=C3C([O-])=O)C%10=CC=C(N%11CCC%11)C=C%101)=[N+]%12CCC/%12

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Hoechst Janelia Fluor^® 646

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	11.19	10

制备储备液 for Hoechst Janelia Fluor^® 646

以下数据基于产品分子量 1119.39。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.1 mM	8.93 mL	44.67 mL	89.33 mL
0.5 mM	1.79 mL	8.93 mL	17.87 mL
1 mM	0.89 mL	4.47 mL	8.93 mL
5 mM	0.18 mL	0.89 mL	1.79 mL

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产品说明书 for Hoechst Janelia Fluor^® 646

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Hoechst Janelia Fluor^® 646

参考文献是支持产品生物活性的出版物。

Legant et al (2016) High density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359 PMID: 26950745

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757

Saphn et al Bleaching-independent, whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores. BioRxiv - Paper not yet peer reviewed

Spahn et al (2019) Whole-Cell, 3D, and multicolor STED imaging with exchangeable fluorophores. Nano Lett. 19 500 PMID: 30525682

Spahn et al (2018) A toolbox for multiplexed super-resolution imaging of the E. coli nucleoid and membrane using novel PAINT labels. Sci. Rep. 8 14768 PMID: 30282984

If you know of a relevant reference for Hoechst Janelia Fluor^® 646, please let us know.

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