Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewKey information: Hoechst Janelia Fluor® 646 is a fluorogenic red-emitting DNA probe. Suitable for fixed and live-cell imaging.
Application:confocal microscopy, super resolution microscopy (SRM) (including PALM and PAINT), lattice light sheet microscopy.
Properties and Photophysical Data: Hoechst Janelia Fluor® 646 preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor® 526 (Cat. No. 7313) for multiplexing experiments. Hoechst Janelia Fluor® 646 can also be combined with Hoechst Janelia Fluor® 526 to perform dual-color STED using the same depletion laser (λdep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor® 646 in E. coli cells (~100 nM - 1 μM). Hoechst Janelia Fluor® 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Excitation and emission maxima (λ) are 655 nm and 670 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1.
Hoechst Janelia Fluor® 646 is fluorogenic and fluoresces only once bound to DNA, enabling hassle-free no-wash experiments.
To measure the absorbance spectrum of Hoechst Janelia Fluo® 646 we recommend the following solvent: TFE plus 0.1% TFA.
Application of Hoechst Janelia Fluor® 646 in U2OS cells. Fixed U2OS cells were stained with Hoechst Janelia Fluor® 646 (1 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 633 nm / emission: 638–759 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer分子量 | 1119.39 |
公式 | C64H70N10O7Si |
储存 | Store at -20°C |
纯度 | ≥85% (HPLC) |
CAS Number | 2462011-60-3 |
InChI Key | DTAPBNBSCGEQMS-UHFFFAOYSA-N |
Smiles | C[Si]1(C)C2=C/C(C=CC2=C(C3=CC(C(NCCOCCOCCNC(CCCOC4=CC=C(C5=NC6=C(C=C(C=C6)C7=NC8=C(C=CC(N9CCN(C)CC9)=C8)N7)N5)C=C4)=O)=O)=CC=C3C([O-])=O)C%10=CC=C(N%11CCC%11)C=C%101)=[N+]%12CCC/%12 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
---|---|---|---|
溶解性 | |||
DMSO | 11.19 | 10 |
以下数据基于产品分子量 1119.39。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
---|---|---|---|
0.1 mM | 8.93 mL | 44.67 mL | 89.33 mL |
0.5 mM | 1.79 mL | 8.93 mL | 17.87 mL |
1 mM | 0.89 mL | 4.47 mL | 8.93 mL |
5 mM | 0.18 mL | 0.89 mL | 1.79 mL |
参考文献是支持产品生物活性的出版物。
Legant et al (2016) High density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359 PMID: 26950745
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757
Saphn et al Bleaching-independent, whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores. BioRxiv - Paper not yet peer reviewed
Spahn et al (2019) Whole-Cell, 3D, and multicolor STED imaging with exchangeable fluorophores. Nano Lett. 19 500 PMID: 30525682
Spahn et al (2018) A toolbox for multiplexed super-resolution imaging of the E. coli nucleoid and membrane using novel PAINT labels. Sci. Rep. 8 14768 PMID: 30282984
If you know of a relevant reference for Hoechst Janelia Fluor® 646, please let us know.
关键词: Hoechst Janelia Fluor 646, Hoechst Janelia Fluor 646 supplier, JF646-Hoechst, Hoechst-JF646, Hoechst, Janelia, Fluor646, fluorogenic, red, emitting, DNA, probes, stains, dyes, live, cell, imaging, ICC, IHC, flow, cytometry, JF, 646, Fluorescent, Stains, Flow, Cytometry, Counterstains, 6804, Tocris Bioscience
引用文献是使用了 Tocris 产品的出版物。
目前没有 Hoechst Janelia Fluor® 646 的引用文献。 您是否知道使用了 Tocris Hoechst Janelia Fluor® 646 的优秀论文? 请告知我们.
目前没有该产品的评论。 Be the first to review Hoechst Janelia Fluor® 646 and earn rewards!
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意,Tocris 仅会向正规科研企业/机构地址发送文献。
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: