Hoechst Janelia Fluor® 646

Pricing Availability   Qty
说明: Fluorogenic, red-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, super resolution microscopy (SRM) (PALM, PAINT), lattice light sheet microscopy
别名: Hoechst JF 646,JF646-Hoechst
化学名: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(4-(4-(5-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate
纯度: ≥85% (HPLC)
说明书
引用文献
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生物活性 for Hoechst Janelia Fluor® 646

Key information: Hoechst Janelia Fluor® 646 is a fluorogenic red-emitting DNA probe. Suitable for fixed and live-cell imaging.

Application:confocal microscopy, super resolution microscopy (SRM) (including PALM and PAINT), lattice light sheet microscopy.

Properties and Photophysical Data: Hoechst Janelia Fluor® 646 preferentially stains and binds minor groove of AT-rich regions. The compound can be combined with fluorogenic green-emitting DNA probe Hoechst Janelia Fluor® 526 (Cat. No. 7313) for multiplexing experiments. Hoechst Janelia Fluor® 646 can also be combined with Hoechst Janelia Fluor® 526 to perform dual-color STED using the same depletion laser (λdep = 775 nm). Sub-diffraction spatial resolution STED imaging can be achieved using Hoechst Janelia Fluor® 646 in E. coli cells (~100 nM - 1 μM). Hoechst Janelia Fluor® 646 is a desirable alternative to large oligonucleotide-conjugated antibodies for PAINT experiments, particularly for bacterial studies. Excitation and emission maxima (λ) are 655 nm and 670 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1.

Hoechst Janelia Fluor® 646 is fluorogenic and fluoresces only once bound to DNA, enabling hassle-free no-wash experiments.

To measure the absorbance spectrum of Hoechst Janelia Fluo® 646 we recommend the following solvent: TFE plus 0.1% TFA.

Scientific Data

Confocal Microscopy Application of Hoechst Janelia Fluor<sup>®</sup> 646 in U2OS cells. View Larger

Application of Hoechst Janelia Fluor® 646 in U2OS cells. Fixed U2OS cells were stained with Hoechst Janelia Fluor® 646 (1 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 633 nm / emission: 638–759 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Hoechst Janelia Fluor® 646

Hoechst Janelia Fluor<sup>®</sup> 646 Dye Spectra
Emission Color Red
Brightness
λabs 655 nm
λem 670 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Cell Permeable Yes
Application Super Resolution Microscopy (PALM, PAINT), Confocal Microscopy, Lattice Light Sheet Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for Hoechst Janelia Fluor® 646

分子量 1119.39
公式 C64H70N10O7Si
储存 Store at -20°C
纯度 ≥85% (HPLC)
CAS Number 2462011-60-3
InChI Key DTAPBNBSCGEQMS-UHFFFAOYSA-N
Smiles C[Si]1(C)C2=C/C(C=CC2=C(C3=CC(C(NCCOCCOCCNC(CCCOC4=CC=C(C5=NC6=C(C=C(C=C6)C7=NC8=C(C=CC(N9CCN(C)CC9)=C8)N7)N5)C=C4)=O)=O)=CC=C3C([O-])=O)C%10=CC=C(N%11CCC%11)C=C%101)=[N+]%12CCC/%12

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Hoechst Janelia Fluor® 646

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 11.19 10

制备储备液 for Hoechst Janelia Fluor® 646

以下数据基于产品分子量 1119.39。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 8.93 mL 44.67 mL 89.33 mL
0.5 mM 1.79 mL 8.93 mL 17.87 mL
1 mM 0.89 mL 4.47 mL 8.93 mL
5 mM 0.18 mL 0.89 mL 1.79 mL

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产品说明书 for Hoechst Janelia Fluor® 646

参考文献 for Hoechst Janelia Fluor® 646

参考文献是支持产品生物活性的出版物。

Legant et al (2016) High density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359 PMID: 26950745

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757

Saphn et al Bleaching-independent, whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores. BioRxiv - Paper not yet peer reviewed

Spahn et al (2019) Whole-Cell, 3D, and multicolor STED imaging with exchangeable fluorophores. Nano Lett. 19 500 PMID: 30525682

Spahn et al (2018) A toolbox for multiplexed super-resolution imaging of the E. coli nucleoid and membrane using novel PAINT labels. Sci. Rep. 8 14768 PMID: 30282984


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