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说明： Inhibits β-catenin-responsive transcription (CRT)
化学名： 5-[[2,5-Dimethyl-1-(3-pyridinyl)-1H-pyrrol-3-yl]methylene]-3-phenyl-2,4-thiazolidinedion
纯度： ≥98% (HPLC)
生物活性 for iCRT 14
iCRT 14 is a potent inhibitor of β-catenin-responsive transcription (CRT) (IC50 = 40.3 nM in assays of Wnt pathway activity). Thought to directly influence the interaction between β-catenin and TCF4. Induces marked G0/G1 cell cycle arrest in HCT-116 and HT29 cell lines. Also acts as transfection enhancer; enhances transgene expression in PC-3 cells by 16 to 35-fold using nonviral systems.
化合物库 for iCRT 14
iCRT 14 is also offered as part of the Tocriscreen 2.0 Max. 了解 Tocris 化合物库的更多信息。
技术数据 for iCRT 14
| 分子量 | 375.44 |
| 公式 | C21H17N3O2S |
| 储存 | Store at +4°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 677331-12-3 |
| PubChem ID | 5967294 |
| InChI Key | NCSHZXNGQYSKLR-UNOMPAQXSA-N |
| Smiles | CC(N(C4=CN=CC=C4)C(C)=C3)=C3/C=C(SC2=O)/C(N2C1=CC=CC=C1)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for iCRT 14
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 28.16 | 75 |
制备储备液 for iCRT 14
以下数据基于产品分子量 375.44。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.75 mM | 3.55 mL | 17.76 mL | 35.51 mL |
| 3.75 mM | 0.71 mL | 3.55 mL | 7.1 mL |
| 7.5 mM | 0.36 mL | 1.78 mL | 3.55 mL |
| 37.5 mM | 0.07 mL | 0.36 mL | 0.71 mL |
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产品说明书 for iCRT 14
参考文献 for iCRT 14
参考文献是支持产品生物活性的出版物。
Gonsalves et al (2011) An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway. Proc.Natl.Acad.Sci.USA 108 5954 PMID: 21393571
Watanabe and Dai (2011) Winning WNT: race to Wnt signaling inhibitors. Proc.Natl.Acad.Sci.USA 108 5929 PMID: 21451134
Spivack et al (2021) Enhancement of transgene expression by the β-catenin inhibitor iCRT14. Plasmid PMID: 33472046
If you know of a relevant reference for iCRT 14, please let us know.
关键词: iCRT 14, iCRT 14 supplier, iCRT14, beta-catenin, b-catenin, β-catenin, catenin-responsive, transcription, crt, inhibitors, inhibits, wnt, signaling, signalling, TCF4, transfection, enhancers, enhances, transgene, expression, plasmid, DNA, polyplex, Beta-catenin, Biochemicals, and, Molecular, Biology, 4299, Tocris Bioscience
9 篇 iCRT 14 的引用文献
引用文献是使用了 Tocris 产品的出版物。 iCRT 14 的部分引用包括：
Brown et al (2019) Aging differentially modulates the Wnt pro-survival signalling pathways in vascular smooth muscle cells. Aging Cell 18 e12844 PMID: 30548452
Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725
Ling et al (2021) Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids. Cell Stem Cell 28 1090-1104.e6 PMID: 33915081
Alex M et al (2021) Heparanase-Induced Activation of AKT Stabilizes β-Catenin and Modulates Wnt/β-Catenin Signaling during Herpes Simplex Virus 1 Infection. MBio 12 e0279221 PMID: 34749529
Wu et al (2012) Differential modulation of the oligodendrocyte transcriptome by sonic hedgehog and bone morphogenetic protein 4 via opposing effects on histone acetylation. J Neurosci 32 6651 PMID: 22573687
Sarah J et al (2023) Pro-inflammatory role of Wnt/β-catenin signaling in endothelial dysfunction. Front Cardiovasc Med 9 1059124 PMID: 36794234
Volpini et al (2018) Trypanosoma cruzi Exploits Wnt Signaling Pathway to Promote Its Intracellular Replication in Macrophages. Front Immunol 9 859 PMID: 29743880
Gowda et al (2018) Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2-β-Catenin-BRG1 Transcriptional Network-Driven CD47 Expression. Mol Cell Biol 38 PMID: 29463646
Paula et al (2020) Developmentally Programmed Tankyrase Activity Upregulates β-Catenin and Licenses Progression of Embryonic Genome Activation. Dev Cell 53 545-560.e7 PMID: 32442396
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iCRT 14 的评论
平均评分: 5 (Based on 1 Review.)
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Useful Wnt inhibitor.
By
Anonymous on 07/11/2020
分析类型： In Vitro
种属： Human
细胞系/组织： HCT116
HCT116 cells were treated with different concentrations of iCRT-14 for 24 hours and the cells were used for transwell assays to investigate the effects of Wnt inhibitor on cell migration.
Pathways for iCRT 14
