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Submit ReviewCHIR 99021 in solution New
Description: Sterile-filtered 10 mM solution of CHIR 99021 pre-dissolved in DMSO
Chemical Name: 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
Purity: ≥97% (HPLC)
Biological Activity for CHIR 99021 in solution
CHIR 99021 in solution is a convenient and ready-to-use 10 mM sterile-filtered solution of CHIR 99021 (Cat. No. 4423) pre-dissolved in DMSO. A potent and highly selective inhibitor of glycogen synthase kinase 3 (GSK-3) (IC50 values are 6.7 and 10 nM for GSK-3β and GSK-3α respectively). Acts as Wnt activator. In combination with the irreversible inhibitor of LSD1, Tranylcypromine (Cat. No. 3852), CHIR 99021 enables reprogramming of mouse embryonic fibroblasts into iPSCs. Enhances mouse and human ESC self-renewal when used in combination with PD 0325901 (Cat. No. 4192); promotes differentiation of hPSCs in cardiomyocytes and allows formation of extended pluripotent stem (EPS) cells in combination with (S)-(+)-Dimethindene maleate (Cat. No. 1425), Minocycline (Cat. No. 3268) and human leukemia inhibitory factor.
CHIR 99021 synthesized to cGMP guidelines also available.
Technical Data for CHIR 99021 in solution
| M. Wt | 465.34 |
| Formula | C22H18Cl2N8 |
| Storage | Store at -20°C |
| Purity | ≥97% (HPLC) |
| CAS Number | 252917-06-9 |
| PubChem ID | 9956119 |
| InChI Key | AQGNHMOJWBZFQQ-UHFFFAOYSA-N |
| Smiles | ClC(C=C1Cl)=CC=C1C2=NC(NCCNC3=CC=C(C#N)C=N3)=NC=C2C4=NC=C(C)N4 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for CHIR 99021 in solution
References for CHIR 99021 in solution
References are publications that support the biological activity of the product.
Ye et al (2012) Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embyronic stem cells from refractory mouse strains. PLoS One 7 e35892 PMID: 22540008
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