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IRDye® 680RD, NHS ester

Cat. No. 8878 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Near-infared dye (reactive group NHS ester)
化学名： Sodium (Z)-2-((2Z,4E)-5-(5-Chloro-3,3-dimethyl-7-(3-sulfonatopropyl)-3H-pyrrolo[2,3-b]pyridin-7-ium-2-yl)-3-(3-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)phenyl)penta-2,4-dien-1-ylidene)-3,3-dimethyl-1-(3-sulfonatopropyl)indoline-5-sulfonate
纯度： ≥70% (HPLC)
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生物活性 技术数据 说明书 参考文献

生物活性 for IRDye® 680RD, NHS ester

Key information: IRDye® 680RD, NHS ester is a near-infrared (NIR) fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines.

Application: Fluorescence microscopy.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 680 nm and 694 nm, respectively.

Please see the protocol for further information on protein/antibody labeling and conjugation.

NOTE: The choice of solvent for the dye is dependent on the stability of the protein in DMSO. Since a small amount of dye is needed to label large (i.e. >45 kDA) proteins, the amount of DMSO in the reaction is negligible and should not affect protein stability. However, since a proportionally larger amount of dye is required to achieve adequate labeling for a small protein (i.e. <45 kDA), the level of DMSO in the reaction is more likely to affect the protein stability. Dye solubilized in water should be discarded after one use. Dye prepared in DMSO is stable for up to two weeks when stored at -20°C and protected from light and moisture.

It is recommended to dissolve dye in water or DMSO (10 - 20 mg/mL)

IRDye® is a registered trademark of Li-Cor and is used under license.

Optical Data for IRDye® 680RD, NHS ester

λabs 680 nm
λem 694 nm

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for IRDye® 680RD, NHS ester

分子量 1003.46
公式 C43H45ClN4Na2O13S3
储存 Store at -20°C
纯度 ≥70% (HPLC)
CAS Number 1373919-87-9
InChI Key JNFTWCVWRDPJNM-UHFFFAOYSA-L
Smiles O=S(C1=CC(C/2(C)C)=C(C=C1)N(CCCS(=O)(O[Na])=O)C2=C/C=C(C3=CC(CCC(ON4C(CCC4=O)=O)=O)=CC=C3)/C=C/C5=NC6=[N+](CCCS([O-])(=O)=O)C=C(Cl)C=C6C5(C)C)(O[Na])=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for IRDye® 680RD, NHS ester

分析证书/产品说明书
化学品安全技术说明书

参考文献 for IRDye® 680RD, NHS ester

参考文献是支持产品生物活性的出版物。

Al-Hawat et al (2024) Development of a polymersome blood ammonia assay coupled with a portable near-infrared fluorometer. ACS Bio.Med.Chem.Au. 4 226 PMID: 39431266

If you know of a relevant reference for IRDye® 680RD, NHS ester, please let us know.

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关键词: IRDye® 680RD, NHS ester, IRDye® 680RD, NHS ester supplier, NIR, near, infrared, short, wave, SWIR, fluorescent, dyes, fluorescence, imaging, labeling, labelling, Near, Infrared, Fluorescent, (NIR), Dyes, 8878, Tocris Bioscience

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