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IRDye® 800CW, Maleimide

Cat. No. 8880 Submit a Review Datasheet / COA / SDS
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Description: Near-infrared dye (Reactive group: maleimide)
Chemical Name: Sodium 2-((E)-2-((E)-3-(2-((E)-1-(6-((2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)ethylidene)-2-(4-sulfonatophenoxy)cyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate
Purity: ≥80% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Technical Data Datasheets References

Biological Activity for IRDye® 800CW, Maleimide

Key information: IRDye® 800CW, Maleimide is a near-infrared (NIR) fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Fluorescence microscopy.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 773 nm and 792 nm, respectively.

Please see the product protocol for further information and a guide to protein/antibody labeling.

It is recommended to dissolve this dye in water or DMSO (10 - 20 mg/mL)

NOTE: When labeling a peptide or protein with IRDye® 800CW Maleimide, the choice of solvent for the dye is dependent on the stability of the target. Since a small amount of dye is needed to label large (i.e. >45 kDa) proteins, the amount of DMSO in the reaction is negligible and should not affect protein stability. However, since a proportionally larger amount of dye is required to achieve adequate labeling for a small protein (i.e. <45 kDa) or peptide, the level of DMSO in the reaction is more likely to affect the target stability. Dye solubilized in aqueous solutions should be discarded after one use. Dye prepared in DMSO is stable when stored at -20 °C (for up to two weeks) and protected from light and moisture.

IRDye® is a registered trademark of Li-Cor and is used under license.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for IRDye® 800CW, Maleimide

M. Wt 1191.25
Formula C52H57N4Na3O16S4
Storage Store at -20°C
Purity ≥80% (HPLC)
CAS Number 1279564-25-8
InChI Key CAXPRNLNWICSJV-UHFFFAOYSA-K
Smiles CC1(C)C2=CC(S(=O)(O[Na])=O)=CC=C2[N+](CCCCS([O-])(=O)=O)=C1/C=C/C3=C(OC4=CC=C(S(=O)(O[Na])=O)C=C4)/C(CCC3)=C/C=C5C(C)(C)C(C=C(S(=O)(O[Na])=O)C=C6)=C6N\5CCCCCC(NCCN7C(C=CC7=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for IRDye® 800CW, Maleimide

Certificate of Analysis / Product Datasheet
Safety Datasheet

References for IRDye® 800CW, Maleimide

References are publications that support the biological activity of the product.

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Citations for IRDye® 800CW, Maleimide

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including Janelia Fluor® Dyes
  • Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
  • Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
  • Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
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