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Submit ReviewIRDye® 800CW, NHS ester New
Description: Near-infared dye (reactive group NHS ester)
Chemical Name: Sodium 2-((E)-2-((E)-3-(2-((E)-1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)ethylidene)-2-(4-sulfonatophenoxy)cyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate
Purity: ≥80% (HPLC)
Biological Activity for IRDye® 800CW, NHS ester
Key information: IRDye® 800CW, NHS ester is a near-infrared (NIR) fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines.
Application: Fluorescence microscopy.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 773 nm and 792 nm, respectively.
Please see the protocol for further information on protein/antibody labeling and conjugation.
It is recommended to dissolve this dye in water or DMSO (10 - 20 mg/mL)
Optical Data for IRDye® 800CW, NHS ester
| λabs | 773 nm |
|---|---|
| λem | 792 nm |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for IRDye® 800CW, NHS ester
| M. Wt | 1166.2 |
| Formula | C50H54N3Na3O17S4 |
| Storage | Store at -20°C |
| Purity | ≥80% (HPLC) |
| CAS Number | 956579-01-4 |
| InChI Key | PBPMREYPAOZRBU-UHFFFAOYSA-K |
| Smiles | CC1(C)C2=CC(S(=O)(O[Na])=O)=CC=C2[N+](CCCCS([O-])(=O)=O)=C1/C=C/C3=C(OC4=CC=C(S(=O)(O[Na])=O)C=C4)/C(CCC3)=C/C=C5C(C)(C)C(C=C(S(=O)(O[Na])=O)C=C6)=C6N\5CCCCCC(ON7C(CCC7=O)=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for IRDye® 800CW, NHS ester
References for IRDye® 800CW, NHS ester
References are publications that support the biological activity of the product.
Sever et al (2025) Detection properties of indium-111 and IRDye800CW for intraoperative molecular imaging use across tissue phantom models. J.Biomed.Opt. S13705 30 PMID: 39310036
If you know of a relevant reference for IRDye® 800CW, NHS ester, please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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