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Janelia Fluor® 525, Maleimide

Cat. No. 8025 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Yellow dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
化学名： 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate
纯度： ≥95% (HPLC)
说明书
引用文献
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生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Janelia Fluor® 525, Maleimide

Key information: Janelia Fluor® 525, Maleimide is a yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 531 nm and 553 nm, respectively.

Please see the product protocol for further information and a guide to protein/antibody labeling.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 525, Maleimide

Emission Color Yellow
Brightness
λabs 531 nm
λem 553 nm
Reactive Group Maleimide
Reactivity Thiols
Cell Permeable Yes
Application Confocal Imaging, dSTORM, STED, Flow Cytometry

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for Janelia Fluor® 525, Maleimide

分子量 648.57
公式 C33H24F4N4O6
储存 Store at -20°C
纯度 ≥95% (HPLC)
InChI Key UUUQGVIAYNYBIT-UHFFFAOYSA-N
Smiles O=C(NCCN1C(C=CC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Janelia Fluor® 525, Maleimide

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 6.49 10

制备储备液 for Janelia Fluor® 525, Maleimide

以下数据基于产品分子量 648.57。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 15.42 mL 77.09 mL 154.19 mL
0.5 mM 3.08 mL 15.42 mL 30.84 mL
1 mM 1.54 mL 7.71 mL 15.42 mL
5 mM 0.31 mL 1.54 mL 3.08 mL

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产品说明书 for Janelia Fluor® 525, Maleimide

分析证书/产品说明书
化学品安全技术说明书

参考文献 for Janelia Fluor® 525, Maleimide

参考文献是支持产品生物活性的出版物。

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods. 12 244 PMID: 25599551

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Janelia Fluor® 525, Maleimide, please let us know.

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