Janelia Fluor^® 549, Azide

Name: Janelia Fluor ® 549, Azide
Brand: Tocris Bioscience
SKU: 6501

Cat. No. 6501 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Discontinued Product

6501 has been discontinued.

说明： Yellow dye (Reactive group: azide). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging

化学名： 3,6-Di-1-azetidinyl-9-[5-[[2-[2-[2-[2-azidoethoxy]ethoxy]ethoxy]ethyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt

纯度： ≥90% (HPLC)

说明书

引用文献

文献 (1)

生物活性技术数据说明书参考文献

生物活性 for Janelia Fluor^® 549, Azide

Janelia Fluor^® 549, Azide is a fluorescent yellow dye; supplied as an azide for click chemisty. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M^-1cm^-1; A280 correction factor is 0.169.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor^® 549, Azide

Janelia Fluor<sup>®</sup> 549, Azide Dye Spectra

Emission Color	Yellow
Brightness
λ_abs	549 nm
λ_em	571 nm
Extinction Coefficient (ε)	101,000 M^-1cm^-1
Quantum Yield (φ)	0.88
Closest Laser line	532 nm
Reactive Group	Azide
Reactivity	Click
Cell Permeable	Yes
Correction Factor 280	0.169
Application	Confocal imaging, dSTORM, STED, Flow
Alternative For	Alexa Fluor^® 546, Alexa Fluor^® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor^® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Janelia Fluor^® 549, Azide

分子量	654.71
公式	C₃₅H₃₈N₆O₇
储存	Store at -20°C
纯度	≥90% (HPLC)
PubChem ID	137919860
InChI Key	XDSXVSGQYCACTI-UHFFFAOYSA-N
Smiles	O=C(NCCOCCOCCOCCN=[N+]=[N-])C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CCC3)C=C2O4)=C(C=C/5)C4=CC5=[N+]6CCC\6)=C1

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for Janelia Fluor^® 549, Azide

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Janelia Fluor^® 549, Azide

参考文献是支持产品生物活性的出版物。

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551

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