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Submit ReviewJanelia Fluor® 549, Tetrazine
说明： Yellow dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
化学名： 3,6-Di-1-azetidinyl-9-[[4-[(1,2,4,5-tetrazin-3-yl)benzyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
生物活性 for Janelia Fluor® 549, Tetrazine
Key Information: Janelia Fluor® 549, Tetrazine is a yellow fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live-cell imaging.
Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Janelia Fluor® 549, tetrazine is cell permeable. It can be coupled directly to a protein of interest using bioorthogonal chemistry and unnatural amino acid technology and has been used in this way to label living primary neurons.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 549, Tetrazine
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Tetrazine |
| Reactivity | Copper-free click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal Microscopy, dSTORM, STED, Live-cell Imaging |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for Janelia Fluor® 549, Tetrazine
| 分子量 | 623.66 |
| 公式 | C36H29N7O4 |
| 储存 | Store at -20°C |
| Smiles | O=C(NCC1=CC=C(C2=NN=CN=N2)C=C1)C3=CC=C(C([O-])=O)C(C(C4=CC=C(N5CCC5)C=C4O6)=C(C=C/7)C6=CC7=[N+]8CCC\8)=C3 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Janelia Fluor® 549, Tetrazine
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 6.24 | 10 |
制备储备液 for Janelia Fluor® 549, Tetrazine
以下数据基于产品分子量 623.66。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 16.03 mL | 80.17 mL | 160.34 mL |
| 0.5 mM | 3.21 mL | 16.03 mL | 32.07 mL |
| 1 mM | 1.6 mL | 8.02 mL | 16.03 mL |
| 5 mM | 0.32 mL | 1.6 mL | 3.21 mL |
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产品说明书 for Janelia Fluor® 549, Tetrazine
参考文献 for Janelia Fluor® 549, Tetrazine
参考文献是支持产品生物活性的出版物。
Arsic et al (2022) Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Nat.Commun. 13 PMID: 35031604
If you know of a relevant reference for Janelia Fluor® 549, Tetrazine, please let us know.
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关键词: Janelia Fluor 549, Tetrazine, Janelia Fluor 549, Tetrazine supplier, JF549, Janelia, Fluor, 549,, Fluorescent, dye, tetrazine, microscopy, cell, permeable, super, resolution, SE, STED, Click, Reactive, Dyes, Click-Reactive, Flow, Cytometry, 6502, Tocris Bioscience
2 篇 Janelia Fluor® 549, Tetrazine 的引用文献
引用文献是使用了 Tocris 产品的出版物。 Janelia Fluor® 549, Tetrazine 的部分引用包括：
Arsic et al (2022) Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Nat.Commun. 13 PMID: 35031604
Yu et al (2023) Visualizing the disordered nuclear transport machinery in situ. Nature 617 162 PMID: 37100914
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals